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Literature summary extracted from

  • Classen, T.; Pietruszka, J.; Schuback, S.
    Revisiting the enantioselective sequence patterns in enoate reductases (2013), ChemCatChem, 5, 711-713.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.31 expressed in Escherichia coli BL21(DE3) cells Photorhabdus luminescens
1.6.99.1 expressed in Escherichia coli BL21(DE3) cells Zymomonas mobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.31 40400
-
-
 Photorhabdus luminescens
1.3.1.31 42500
-
 x * 42500, SDS-PAGE Photorhabdus luminescens
1.6.99.1 32600
-
-
 Zymomonas mobilis
1.6.99.1 41700
-
 x * 41700, calculated from amino acid sequence Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Photorhabdus luminescens
-
 subspecies Photorhabdus luminescens laumondii
-
1.3.1.31 Solanum lycopersicum
-
-
-
1.6.99.1 Zymomonas mobilis
-
-
-
1.6.99.1 Zymomonas mobilis ATCC 29191
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.31 Ni-NTA column chromatography Photorhabdus luminescens
1.6.99.1 Ni-NTA column chromatography Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Photorhabdus luminescens ?
-
 ?
1.3.1.31 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Solanum lycopersicum [(2R)-1-nitropropan-2-yl]benzene + NADPH + H+ product of isoform OPR1 ?
1.3.1.31 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Photorhabdus luminescens [(2S)-1-nitropropan-2-yl]benzene + NADPH + H+
-
 ?
1.3.1.31 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Solanum lycopersicum [(2S)-1-nitropropan-2-yl]benzene + NADPH + H+ product of isoform OPR3 ?
1.3.1.31 (E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
-
 Photorhabdus luminescens ?
-
 ?
1.3.1.31 (E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
-
 Photorhabdus luminescens [(3S)-3-nitrobutyl]benzene + NADPH + H+
-
 ?
1.3.1.31 (E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+
-
 Photorhabdus luminescens ?
-
 ?
1.3.1.31 (E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+
-
 Photorhabdus luminescens ethyl (2R)-2-methyl-4-oxopentanoate + NADPH + H+
-
 ?
1.3.1.31 methyl 2-phenylacrylate + NADP+
-
 Photorhabdus luminescens methyl (2R)-2-methyl-3-phenylpropanoate + NADPH + H+
-
 ?
1.3.1.31 methyl 2-phenylacrylate + NADP+
-
 Photorhabdus luminescens ?
-
 ?
1.6.99.1 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Zymomonas mobilis ?
-
 ?
1.6.99.1 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Zymomonas mobilis ATCC 29191 ?
-
 ?
1.6.99.1 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Zymomonas mobilis [(2S)-1-nitropropan-2-yl]benzene + NADPH + H+
-
 ?
1.6.99.1 (1-nitroprop-1-en-2-yl)benzene + NADP+
-
 Zymomonas mobilis ATCC 29191 [(2S)-1-nitropropan-2-yl]benzene + NADPH + H+
-
 ?
1.6.99.1 (E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
-
 Zymomonas mobilis ?
-
 ?
1.6.99.1 (E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
-
 Zymomonas mobilis ATCC 29191 ?
-
 ?
1.6.99.1 (E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
-
 Zymomonas mobilis [(3S)-3-nitrobutyl]benzene + NADPH + H+
-
 ?
1.6.99.1 (E)-(2-nitroprop-1-en-1-yl)benzene + NADP+
-
 Zymomonas mobilis ATCC 29191 [(3S)-3-nitrobutyl]benzene + NADPH + H+
-
 ?
1.6.99.1 (E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+
-
 Zymomonas mobilis ?
-
 ?
1.6.99.1 (E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+
-
 Zymomonas mobilis ATCC 29191 ?
-
 ?
1.6.99.1 (E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+
-
 Zymomonas mobilis ethyl (2R)-2-methyl-4-oxopentanoate + NADPH + H+
-
 ?
1.6.99.1 methyl 2-phenylacrylate + NADP+
-
 Zymomonas mobilis methyl (2R)-2-methyl-3-phenylpropanoate + NADPH + H+
-
 ?
1.6.99.1 methyl 2-phenylacrylate + NADP+
-
 Zymomonas mobilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.31 ? x * 42500, SDS-PAGE Photorhabdus luminescens
1.3.1.31 ? x * 40400, calculated from amino acid sequence Photorhabdus luminescens
1.6.99.1 ? x * 41700, calculated from amino acid sequence Zymomonas mobilis
1.6.99.1 ? x * 32600, SDS-PAGE Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
1.3.1.31 NCR
-
 Photorhabdus luminescens
1.3.1.31 OPR1
-
 Solanum lycopersicum
1.3.1.31 OPR3
-
 Solanum lycopersicum
1.6.99.1 NADPH-dependent cyclohexenone reductase
-
 Zymomonas mobilis
1.6.99.1 NCR
-
 Zymomonas mobilis
1.6.99.1 YqjM
-
 Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.31 NADP+
-
 Photorhabdus luminescens
1.3.1.31 NADP+
-
 Solanum lycopersicum
1.6.99.1 NADP+
-
 Zymomonas mobilis