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Literature summary extracted from

  • Knop, M.; Engi, P.; Lemnaru, R.; Seebeck, F.P.
    In Vitro Reconstitution of Formylglycine-Generating Enzymes Requires Copper(I) (2015), ChemBioChem, 16, 2147-2150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.7
-
 Mycolicibacterium smegmatis
1.8.3.7
-
 Thermomonospora curvata

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.3.7
-
 Thermomonospora curvata

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.7 C187A/C231A/C284S/C298A ten-fold increased activity compared to wild-type Thermomonospora curvata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.7 Cu+ CuSO4 and CuCl are almost equally efficient Mycolicibacterium smegmatis
1.8.3.7 Cu+ CuSO4 and CuCl are almost equally efficient Thermomonospora curvata
1.8.3.7 Cu2+ CuSO4 and CuCl are almost equally efficient Mycolicibacterium smegmatis
1.8.3.7 Cu2+ CuSO4 and CuCl are almost equally efficient Thermomonospora curvata

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.7 Mycolicibacterium smegmatis
-
-
-
1.8.3.7 Mycolicibacterium smegmatis DSM 43465
-
-
-
1.8.3.7 Thermomonospora curvata
-
-
-
1.8.3.7 Thermomonospora curvata DSM 43183
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.7 Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
 Mycolicibacterium smegmatis Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
 ?
1.8.3.7 Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
 Thermomonospora curvata Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
 ?
1.8.3.7 Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
 Mycolicibacterium smegmatis DSM 43465 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
 ?
1.8.3.7 Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
 Thermomonospora curvata DSM 43183 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.7 FGE
-
 Mycolicibacterium smegmatis
1.8.3.7 FGE
-
 Thermomonospora curvata

Temperature Stability [掳C]

EC Number Temperature Stability Minimum [掳C] Temperature Stability Maximum [掳C] Comment Organism
1.8.3.7 43
-
 mutant enzyme C187A/C231A/C284S/C298A, stable up to Thermomonospora curvata
1.8.3.7 51
-
 wild-type enzyme, stable up to Thermomonospora curvata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.7 0.01
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 Thermomonospora curvata
1.8.3.7 0.027
-
 Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata
1.8.3.7 0.07
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.3.7 1.7
-
 Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 Thermomonospora curvata
1.8.3.7 17
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 Thermomonospora curvata
1.8.3.7 48.3
-
 Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata
1.8.3.7 333.3
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata