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Literature summary extracted from

  • Hamre, A.G.; Eide, K.B.; Wold, H.H.; Sorlie, M.
    Activation of enzymatic chitin degradation by a lytic polysaccharide monooxygenase (2015), Carbohydr. Res., 407, 166-169.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53 expression in Escherichia coli Serratia marcescens
3.2.1.14 expression in Escherichia coli Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Serratia marcescens O83009
-
-
3.2.1.14 Serratia marcescens P07254
-
-
3.2.1.14 Serratia marcescens Q54276
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 beta-chitin + H2O substrate 3 micrometer beta-chitin Serratia marcescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.53 CBP21
-
 Serratia marcescens
3.2.1.14 ChiA
-
 Serratia marcescens
3.2.1.14 ChiB
-
 Serratia marcescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 1.7
-
 beta-chitin pH 8.0, 37°C Serratia marcescens
3.2.1.14 2.2
-
 beta-chitin pH 6.1, 37°C Serratia marcescens
3.2.1.14 9.2
-
 beta-chitin presence of lytic polysaccharide monooxygenase Cbp21, pH 6.1, 37°C Serratia marcescens
3.2.1.14 11.1
-
 beta-chitin presence of lytic polysaccharide monooxygenase Cbp21, pH 8.0, 37°C Serratia marcescens
3.2.1.14 13.9
-
 beta-chitin presence of lytic polysaccharide monooxygenase Cbp21, pH 8.0, 37°C Serratia marcescens

General Information

EC Number General Information Comment Organism
1.14.99.53 physiological function in presence of lytic polysaccharide monooxygenase Cbp21, the apparent kcat values of chitinases ChiA and ChiB increase 6-9fold, while there is no effect on chitinase ChiC Serratia marcescens
3.2.1.14 physiological function in presence of lytic polysaccharide monooxygenase Cbp21, the apparent kcat values of chitinases ChiA and ChiB increase 6-9fold, while there is no effect on chitinase ChiC Serratia marcescens