Literature summary extracted from
Martinez-Rocha, A.L.; Woriedh, M.; Chemnitz, J.; Willingmann, P.; Kroeger, C.; Hadeler, B.; Hauber, J.; Schaefer, W.
Posttranslational hypusination of the eukaryotic translation initiation factor-5A regulates Fusarium graminearum virulence (2016), Sci. Rep., 6, 24698.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.46 |
gene DHS, targeted enzyme overexpression in Fusarium graminearum by single crossover event inserting the linearized vector pMW-DHS |
Fusarium graminearum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.5.1.46 |
additional information |
replacement of the endogenous DHS and DOHH genes by homologous recombination with the PgpdA-DHS or Pgpd1-DOHH alleles, excluding any possibility of mutagenic effects by ectopic integration |
Fusarium graminearum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.5.1.46 |
CNI-1493 |
a compound that inhibits fungal DHS activity without affecting grain development |
Fusarium graminearum |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.5.1.46 |
[eIF5A-precursor]-lysine + spermidine |
Fusarium graminearum |
hypusination of Lys56 |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.46 |
Fusarium graminearum |
D5L6Z2 |
i.e. Gibberella zeae, gene DHS |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.46 |
[eIF5A-precursor]-lysine + spermidine |
hypusination of Lys56 |
Fusarium graminearum |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.46 |
DHS |
- |
Fusarium graminearum |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.5.1.46 |
malfunction |
importance of imbalances in eIF5A hypusination in Fusarium graminearum: overexpression of gene DHS leads to increased virulence in Triticum aestivum, elevated production of the mycotoxin deoxynivalenol, more infection structures, faster Triticum aestivum tissue invasion in plants and increases vegetatively produced conidia. Overexpression of the enzyme DOHH completely prevents infection structure formation, pathogenicity in Triticum aestivum and Zea mays, leads to overproduction of reactive oxygen species, reduced DON mycotoxin production and increased sexual reproduction. Simultaneous overexpression of both genes restores wild type-like phenotypes, strongly increased hypusinated eIF5A in DOHH overexpression mutant in comparison to wild-type, and the DHS overexpression mutants |
Fusarium graminearum |
2.5.1.46 |
physiological function |
activation of eukaryotic translation initiation factor eIF5A requires a posttranslational modification, forming the unique amino acid hypusine. This activation is mediated by two enzymes, deoxyhypusine synthase, DHS, and deoxyhypusine hydroxylase, DOHH. Enzyme DHS catalyzes eIF5A Lys56 hypusination and is essential in Fusarium graminearum |
Fusarium graminearum |