Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nishimura, M.
    Molecular cloning and expression of the Streptomyces coniferyl alcohol dehydrogenase gene in Escherichia coli (2013), Protein Expr. Purif., 89, 109-115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.90 DNA and amino acid sequence determination and analysis, sequence comparisons, functional recombinant enzyme overexpression in Escherichia coli strain BL21(DE3), optimization of conditions and method for soluble CADH expression, expression of active CADH under optimized conditions is approximately 4fold higher than in the absence of heat shock, solution of inclusion body formation by heat-shock treatment of recombinant Escherichia coli carrying the Streptomyces CADH gene Streptomyces sp.
1.1.1.194 expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.13
-
 coniferyl alcohol native enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.90 0.28
-
 coniferyl alcohol recombinant enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.194 0.28
-
 coniferyl alcohol recombinant enzyme, at pH 8.5 and 40°C Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.90 Zn2+ required Streptomyces sp.
1.1.1.194 Zn2+ dependent on Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 150000
-
 recombinant enzyme, gel filtration Streptomyces sp.
1.1.1.194 39000
-
 4 * 39000, SDS-PAGE Streptomyces sp.
1.1.1.194 150000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 coniferyl alcohol + NAD+ Streptomyces sp. CADH requires NAD as a cofactor coniferyl aldehyde + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Streptomyces sp. M5AJW4
-
-
1.1.1.194 Streptomyces sp. M5AJW4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.90 recombinant enzyme about 40fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, hydrophobic interaction chromatography, again dialysis, and anion exchange chromatography Streptomyces sp.
1.1.1.194 ammonium sulfate precipitation, Resource PHE column chromatography, and Mono Q Superdex 200 column chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.90 10.7
-
 puriifed recombinant enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.194 0.27
-
 cell-free extract, at pH 8.5 and 40°C Streptomyces sp.
1.1.1.194 10.7
-
 after 40fold purification, at pH 8.5 and 40°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 coniferyl alcohol + NAD+ CADH requires NAD as a cofactor Streptomyces sp. coniferyl aldehyde + NADH + H+
-
 ?
1.1.1.194 coniferyl alcohol + NADP+
-
 Streptomyces sp. coniferyl aldehyde + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.90 homotetramer 4* 39000, recombinant and native enzyme, SDS-PAGE, 4* 37845, sequence calculation Streptomyces sp.
1.1.1.194 homotetramer 4 * 39000, SDS-PAGE Streptomyces sp.
1.1.1.194 homotetramer 4 * 37845, calculated from amino acid sequence Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.90 CADH
-
 Streptomyces sp.
1.1.1.90 coniferyl alcohol dehydrogenase
-
 Streptomyces sp.
1.1.1.194 CADH
-
 Streptomyces sp.
1.1.1.194 coniferyl alcohol dehydrogenase
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.90 30
-
 assay at Streptomyces sp.
1.1.1.194 40
-
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 25.8
-
 coniferyl alcohol recombinant enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.90 69.7
-
 coniferyl alcohol native enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.194 25.8
-
 coniferyl alcohol recombinant enzyme, at pH 8.5 and 40°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 8.5
-
 assay at Streptomyces sp.
1.1.1.194 8.5
-
-
 Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Streptomyces sp.
1.1.1.194 NADP+
-
 Streptomyces sp.

General Information

EC Number General Information Comment Organism
1.1.1.90 evolution the enzyme belongs to the medium-chain zinc-dependent alcohol dehydrogenases Streptomyces sp.
1.1.1.90 physiological function the enzyme is a medium-chain zinc-dependent alcohol dehydrogenase Streptomyces sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.90 93.3
-
 coniferyl alcohol recombinant enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.90 536
-
 coniferyl alcohol native enzyme, pH 8.5, 30°C Streptomyces sp.
1.1.1.194 93.3
-
 coniferyl alcohol recombinant enzyme, at pH 8.5 and 40°C Streptomyces sp.