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Literature summary extracted from

  • Zhao, Y.J.; Chen, X.; Zhang, M.; Su, P.; Liu, Y.J.; Tong, Y.R.; Wang, X.J.; Huang, L.Q.; Gao, W.
    Molecular cloning and characterisation of farnesyl pyrophosphate synthase from Tripterygium wilfordii (2015), PLoS ONE, 10, e0125415.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 expressed in Escherichia coli BL21(DE3) cells Tripterygium wilfordii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 58000
-
-
 Tripterygium wilfordii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Tripterygium wilfordii
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Tripterygium wilfordii A0A0F6PMF9
-
-
2.5.1.10 Tripterygium wilfordii A0A0F6PN04
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 Ni-NTA column chromatography Tripterygium wilfordii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.10 leaf
-
 Tripterygium wilfordii
-
2.5.1.10 root high expression Tripterygium wilfordii
-
2.5.1.10 stem
-
 Tripterygium wilfordii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Tripterygium wilfordii diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.10 ? x * 39540, calculated from amino acid sequence Tripterygium wilfordii
2.5.1.10 ? x * 58000, enzyme fused with a Trx-tag and a His-tag on N-terminal Tripterygium wilfordii

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 farnesyl pyrophosphate synthase
-
 Tripterygium wilfordii
2.5.1.10 Fps1 isoform Tripterygium wilfordii
2.5.1.10 FPS2 isoform Tripterygium wilfordii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.10 Tripterygium wilfordii isoform FPS2, calculated from amino acid sequence
-
 5.3
2.5.1.10 Tripterygium wilfordii isoform FPS1, calculated from amino acid sequence
-
 5.6

Expression

EC Number Organism Comment Expression
2.5.1.10 Tripterygium wilfordii the enzyme expression is up-regulated upon 1 mM methyl jasmonate treatment after 12 h up