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Literature summary extracted from

  • Brasher, M.I.; Surmacz, L.; Leong, B.; Pitcher, J.; Swiezewska, E.; Pichersky, E.; Akhtar, T.A.
    A two-component enzyme complex is required for dolichol biosynthesis in tomato (2015), Plant J., 82, 903-914.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.87 gene rer2, phylogenetic analysis, quantitative RT-PCR expression analysis, functional complementation of the yeast rer2DELTA dolichol mutant, production of dolichols in rer2DELTA strains expressing both SlCPT3 and SlCPTBP associating together in an enzyme complex, recombinant coexpression of C-terminally Myc-tagged SlCPT3 and FLAG-tagged SlCPTBP in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain LBA4404, recombinant expression of C-terminally Myc-tagged SlCPT3 in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.87 additional information generation of RNAi-mediated knockdown of SlCPT3 expression in plants, SlCPT3 gene expression and also polyisoprenoid contents are reduced by approximately 60% and 40%, respectively. Although transgenic plantlets are recovered that exhibit a higher degree of SlCPT3 knockdown, they do snot survive to maturity. Interaction analysis in planta of SlCPT3 and SlCPTBP proteins by introducing C-terminally Myc-tagged SlCPT3 and FLAG-tagged SlCPTBP versions into Nicotiana benthamiana leaves in the endoplasmic reticulum lumeninal side Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.87 endoplasmic reticulum membrane luminal side Solanum lycopersicum 5789
-
2.5.1.87 microsome
-
 Solanum lycopersicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.87 Mg2+ required Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate Solanum lycopersicum
-
 n diphosphate + ditrans,polycis-polyprenyl diphosphate n = 10-55 ?
2.5.1.87 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate Solanum lycopersicum MP-1
-
 n diphosphate + ditrans,polycis-polyprenyl diphosphate n = 10-55 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.87 Solanum lycopersicum K7WCI9
-
-
2.5.1.87 Solanum lycopersicum MP-1 K7WCI9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate
-
 Solanum lycopersicum n diphosphate + ditrans,polycis-polyprenyl diphosphate n = 10-55 ?
2.5.1.87 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate
-
 Solanum lycopersicum MP-1 n diphosphate + ditrans,polycis-polyprenyl diphosphate n = 10-55 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.87 cis-prenyltransferase
-
 Solanum lycopersicum
2.5.1.87 SlCPT3
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.87 22
-
 assay at room temperature Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.87 8
-
 assay at Solanum lycopersicum

General Information

EC Number General Information Comment Organism
2.5.1.87 evolution the class of enzymes known as cis-prenyltransferases (CPTs) elongate the trans-prenyl diphosphate intermediate with a cis-linked linear polymer of isopentenyl diphosphate units Solanum lycopersicum
2.5.1.87 physiological function the SlCPT3 and SlCPTBP proteins interact to form a functional dolichol synthase Solanum lycopersicum