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Literature summary extracted from

  • Mittal, N.; Morada, M.; Tripathi, P.; Gowri, V.S.; Mandal, S.; Quirch, A.; Park, M.H.; Yarlett, N.; Madhubala, R.
    Cryptosporidium parvum has an active hypusine biosynthesis pathway (2014), Mol. Biochem. Parasitol., 195, 14-22.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.46 drug development Cryptosporidium parvum DHS is a traget for drug development, as GC7 effectively inhibits parasite infection and growth in cultured host cells Cryptosporidium parvum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.46 gene DHS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Cryptosporidium parvum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.46 1,8-diaminooctane weaker inhibition Cryptosporidium parvum
2.5.1.46 diaminononane weaker inhibition Cryptosporidium parvum
2.5.1.46 N1-guanyl-1,7-diaminoheptane GC7, a potent competitive inhibitor of DHS, causes an effective inhibition of infection and growth of Cryptosporidium parvum in human HCT-8 adenocarcinoma cells. Complete inhibition at 0.01 mM Cryptosporidium parvum
2.5.1.46 paromomycin inhibits oocysts formation Cryptosporidium parvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.46 0.00091
-
 [eIF5A-precursor]-lysine pH 7.5, 37°C, heterotetrameric DHS Cryptosporidium parvum
2.5.1.46 0.0123
-
 spermidine pH 7.5, 37°C, heterotetrameric DHS Cryptosporidium parvum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 40800
-
-
 Cryptosporidium parvum
2.5.1.46 180000
-
 native PAGE Cryptosporidium parvum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Cryptosporidium parvum
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Cryptosporidium parvum A3FQA5 single-copy gene DHS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography Cryptosporidium parvum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Cryptosporidium parvum [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.46 tetramer 4 * 40800, about, sequence calculation Cryptosporidium parvum

Synonyms

EC Number Synonyms Comment Organism
2.5.1.46 CpDHS
-
 Cryptosporidium parvum
2.5.1.46 DHS
-
 Cryptosporidium parvum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.46 37
-
 assay at Cryptosporidium parvum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.46 9.2
-
 assay at Cryptosporidium parvum

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+ required Cryptosporidium parvum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.46 additional information
-
 additional information inhibition profile in a series of kinetic experiments Cryptosporidium parvum

General Information

EC Number General Information Comment Organism
2.5.1.46 evolution CpDHS is more closely related to apicomplexan and human DHS than to kinetoplast DHS Cryptosporidium parvum
2.5.1.46 additional information enzyme structure modelling, model evaluation and comparative structural analysis, overview Cryptosporidium parvum
2.5.1.46 physiological function hypusination of eIF5A is an important post-translational modification essential for cell proliferation. This modification occurs in a two step processcatalyzed by deoxyhypusine synthase followed by deoxyhypusine hydroxylase Cryptosporidium parvum