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Literature summary extracted from

  • Bastian, S.; Liu, X.; Meyerowitz, J.; Snow, C.; Chen, M.; Arnold, F.
    Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli (2011), Metab. Eng., 13, 345-352.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.383 biofuel production Recent work has demonstrated glucose to isobutanol conversion through a modified amino acid pathway in a recombinant organism. We demonstrate that an NADH-dependent pathway enables anaerobic isobutanol production at 100% theoretical yield and at higher titer and productivity than both the NADPH-dependent pathway and transhydrogenase over-expressing strain Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.383 A71S mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 A71S/R76D/S78D/Q110A mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 A71S/R76D/S78D/Q110V mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 Q110A mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 Q110V mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 R76D mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 S78D mutant engineered to use NADH instead of NADPH Escherichia coli
1.1.1.383 S78D/Q110V mutant engineered to use NADH instead of NADPH Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.383 0.013
-
 NADPH mutant Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.024
-
 NADPH mutant Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.03
-
 NADH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.04
-
 NADPH wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.04
-
 NADH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.065
-
 NADPH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.065
-
 NADH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.13
-
 NADH mutant S78D, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.14
-
 NADH mutant Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.28
-
 NADH mutant Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.57
-
 NADPH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.65
-
 NADPH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.66
-
 NADPH mutant S78D, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 1.08
-
 NADH wild-type, pH 7.0, temperature not specified in the publication Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.383 Escherichia coli P05793
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
 Escherichia coli 2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
 Escherichia coli 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.383 0.2
-
 NADPH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.3
-
 NADH wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.6
-
 NADPH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 2
-
 NADH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 2.2
-
 NADPH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 2.3
-
 NADH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 2.5
-
 NADPH mutant S78D, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 2.6
-
 NADH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 3.1
-
 NADH mutant Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 3.3
-
 NADH mutant Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 3.6
-
 NADPH wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 3.6
-
 NADH mutant S78D, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 7.2
-
 NADPH mutant Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 7.2
-
 NADPH mutant Q110V, pH 7.0, temperature not specified in the publication Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.383 0.3
-
 NADH wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 0.5
-
 NADPH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 1.1
-
 NADPH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 3.8
-
 NADPH mutant S78D, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 11
-
 NADH mutant Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 24.8
-
 NADH mutant Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 27.6
-
 NADH mutant S78D, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 31.8
-
 NADH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 34.2
-
 NADPH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 60.3
-
 NADH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 74
-
 NADH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 87.3
-
 NADPH wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 302
-
 NADPH mutant Q110A, pH 7.0, temperature not specified in the publication Escherichia coli
1.1.1.383 569
-
 NADPH mutant Q110V, pH 7.0, temperature not specified in the publication Escherichia coli