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Literature summary extracted from

  • Deng, L.X.; Shen, Y.M.; Song, S.Y.
    Cloning, expression, and characterization of para-aminobenzoic acid (PABA) synthase from Agaricus bisporus 02, a thermotolerant mushroom strain (2015), J. Microbiol. Biotechnol., 25, 66-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.85 expression in Escherichia coli Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.85 0.117
-
 chorismate pH 8.0, 25°C Agaricus bisporus
2.6.1.85 2.4
-
 L-glutamine pH 8.0, 25°C Agaricus bisporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.85 Co2+ divalent cation required, in decreasing activity: Mg2+, Mn2+, Co2+ Agaricus bisporus
2.6.1.85 Mg2+ divalent cation required, in decreasing activity: Mg2+, Mn2+, Co2+. Highest activity at 5 mM Mg2+ Agaricus bisporus
2.6.1.85 Mn2+ divalent cation required, in decreasing activity: Mg2+, Mn2+, Co2+ Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.85 80600
-
-
 Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Agaricus bisporus A0A060IGN1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 chorismate + L-asparagine 11% of the activity with L-glutamine Agaricus bisporus 4-amino-4-deoxychorismate + L-aspartate
-
 ?
2.6.1.85 chorismate + L-glutamine
-
 Agaricus bisporus 4-amino-4-deoxychorismate + L-glutamate
-
 ?
2.6.1.85 chorismate + NH3 30% of the activity with L-glutamine Agaricus bisporus 4-amino-4-deoxychorismate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.85 ? x * 80600, calculated Agaricus bisporus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.85 PabAB
-
 Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.85 25
-
-
 Agaricus bisporus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.85 0.013
-
 L-glutamine pH 8.0, 25°C Agaricus bisporus
2.6.1.85 0.017
-
 chorismate pH 8.0, 25°C Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.85 8
-
-
 Agaricus bisporus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.85 5.1
-
 L-glutamine pH 8.0, 25°C Agaricus bisporus
2.6.1.85 143
-
 chorismate pH 8.0, 25°C Agaricus bisporus