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Literature summary extracted from

  • Allen, S.J.; Parthasarathy, G.; Darke, P.L.; Diehl, R.E.; Ford, R.E.; Hall, D.L.; Johnson, S.A.; Reid, J.C.; Rickert, K.W.; Shipman, J.M.; Soisson, S.M.; Zuck, P.; Munshi, S.K.; Lumb, K.J.
    Structure and function of the hypertension variant A486V of G protein-coupled receptor kinase 4 (2015), J. Biol. Chem., 290, 20360-20373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.16 expressed in Sf21 insect cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.11.16 mutant enzyme A486V crystallized in the presence of 5'-adenylyl beta,gamma-imidodiphosphate, sitting drop vapor diffusion method, at 4°C in 0.1 M sodium citrate, pH 5.0-6.5, 15-27.5% (w/v) polyethylene glycol 3350 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.16 A142V the mutation is associated with hypertension Homo sapiens
2.7.11.16 A486V the mutation is associated with hypertension. There is an increased rate of autophosphorylation of a number of residues in the mutant relative to the wild type enzyme Homo sapiens
2.7.11.16 R65L the mutation is associated with hypertension Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.16 ATP + [G-protein-coupled receptor] Homo sapiens
-
 ADP + [G-protein-coupled receptor]phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.16 Homo sapiens P32298
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.16 HisTrap column chromatography, HiTrap SP column chromatography, HiTrap heparin column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.16 ATP + [G-protein-coupled receptor]
-
 Homo sapiens ADP + [G-protein-coupled receptor]phosphate
-
 ?
2.7.11.16 additional information autophosphorylation of wild type isoform GRK4alpha is required for full kinase activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.16 G protein-coupled receptor kinase 4
-
 Homo sapiens
2.7.11.16 GRK4
-
 Homo sapiens