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Literature summary extracted from

  • Rajpurohit, Y.; Misra, H.
    Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans (2013), Int. J. Biochem. Cell Biol., 45, 2541-2552.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expressed in Escherichia coli BL21 (DE3) pLysS cells Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 K42A inactive Deinococcus radiodurans
2.7.11.1 S171A the mutant shows about 10% of wild type activity Deinococcus radiodurans
2.7.11.1 T169A the mutant shows about 5% of wild type activity Deinococcus radiodurans
2.7.11.1 T169A/S171A the mutant shows less than 1% of wild type activity Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + PprA protein Deinococcus radiodurans phosphorylation at threonine 72, serine 112, and threonine 144 ADP + PprA phosphoprotein
-
 ?
2.7.11.1 ATP + PprA protein Deinococcus radiodurans ATCC 13939 phosphorylation at threonine 72, serine 112, and threonine 144 ADP + PprA phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Deinococcus radiodurans Q9RRH3
-
-
2.7.11.1 Deinococcus radiodurans ATCC 13939 Q9RRH3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 nickel affinity column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + PprA protein phosphorylation at threonine 72, serine 112, and threonine 144 Deinococcus radiodurans ADP + PprA phosphoprotein
-
 ?
2.7.11.1 ATP + PprA protein phosphorylation at threonine 72, serine 112, and threonine 144 Deinococcus radiodurans ATCC 13939 ADP + PprA phosphoprotein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 DR2518 locus name Deinococcus radiodurans
2.7.11.1 RqkA
-
 Deinococcus radiodurans

General Information

EC Number General Information Comment Organism
2.7.11.1 physiological function serine/threonine phosphorylation of PprA protein by the enzyme supports its role in radioresistance of Deinococcus radiodurans Deinococcus radiodurans