Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murphy, J.; Mullins, E.; Kappock, T.
    Functional dissection of the bipartite active site of the class I coenzyme A (CoA)-transferase succinyl-CoA:acetate CoA-transferase (2016), Front. Chem., 4, 23.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.18 expressed in Escherichia coli C41(DE3) cells Acetobacter aceti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.3.18 enzyme bound to dethiaacetyl-CoA and acetate, hanging drop vapor diffusion method, using 0.9 M sodium citrate, 0.1 M imidazole-HCl, pH 8.2, and 25 mM 2-mercaptoethanol Acetobacter aceti

Protein Variants

EC Number Protein Variants Comment Organism
2.8.3.18 N347A the mutant retains about 15% of wild type specific activity Acetobacter aceti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.18 succinyl-CoA + acetate Acetobacter aceti
-
 acetyl-CoA + succinate
-
 ?
2.8.3.18 succinyl-CoA + acetate Acetobacter aceti 1023
-
 acetyl-CoA + succinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.18 Acetobacter aceti B3EY95
-
-
2.8.3.18 Acetobacter aceti 1023 B3EY95
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.18 succinyl-CoA + acetate
-
 Acetobacter aceti acetyl-CoA + succinate
-
 ?
2.8.3.18 succinyl-CoA + acetate
-
 Acetobacter aceti 1023 acetyl-CoA + succinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.3.18 AarC
-
 Acetobacter aceti
2.8.3.18 succinyl-CoA:acetate CoA-transferase
-
 Acetobacter aceti