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Literature summary extracted from

  • Chen, X.; Li, M.; Zhou, L.; Shen, W.; Algasan, G.; Fan, Y.; Wang, Z.
    Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose (2014), Biores. Technol., 166, 64-71.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.2 gene ppsA, subcloning in Escherichia coli strain JM19, recombinant overexpression in Escherichia coli strain B0013, coexpression with genes aroG and tktA Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.2 additional information three critical enzymes of mutated 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase (encoded by aroGfbr), PEP synthase (encoded by ppsA), and transketolase A (encoded by tktA) are modularly overexpressed in Escherichia coli and the resulting recombinant strain produces increased levels of shikimate in an optimized fed-batch fermentation process, overview Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.2 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.2 ATP + pyruvate + H2O Escherichia coli
-
 AMP + phosphoenolpyruvate + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.2 Escherichia coli P23538 B0013, gene ppsA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.2 ATP + pyruvate + H2O
-
 Escherichia coli AMP + phosphoenolpyruvate + phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.9.2 PEP synthase
-
 Escherichia coli
2.7.9.2 phosphoenolpyruvate synthase
-
 Escherichia coli
2.7.9.2 PpsA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.2 ATP
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.9.2 metabolism phosphoenolpyruvate produced by the enzyme is required in the shikimate biosynthesis, pathway overview. The expression levels of the enzyme transketolase I (encoded by tktA) and phosphoenolpyruvate synthase (encoded by ppsA) have a critical impact on the phosphoenolpyruvate and erythrose-4-phosphate availability, which are important determinants for in vivo 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase activity Escherichia coli