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Literature summary extracted from

  • Mishra, A.K.; Agnihotri, P.; Srivastava, V.K.; Pratap, J.V.
    Novel protein-protein interaction between spermidine synthase and S-adenosylmethionine decarboxylase from Leishmania donovani (2015), Biochem. Biophys. Res. Commun., 456, 637-642.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expressed in Escherichia coli BL21(DE3) cells Leishmania donovani
4.1.1.50 expressed in Escherichia coli BL21(DE3) cells Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 33000
-
 x * 33000, SDS-PAGE Leishmania donovani
4.1.1.50 35000
-
 2 * 35000, SDS-PAGE Leishmania donovani
4.1.1.50 68000
-
 gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine Leishmania donovani
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?
4.1.1.50 S-adenosyl-L-methionine Leishmania donovani
-
 S-adenosyl 3-(methylthio)propylamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Leishmania donovani A4HS53
-
-
4.1.1.50 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 immobilized metal affinity column chromatography Leishmania donovani
4.1.1.50 GST affinity column chromatography Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine
-
 Leishmania donovani S-methyl-5'-thioadenosine + spermidine
-
 ?
4.1.1.50 S-adenosyl-L-methionine
-
 Leishmania donovani S-adenosyl 3-(methylthio)propylamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 ? x * 33000, SDS-PAGE Leishmania donovani
4.1.1.50 homodimer 2 * 35000, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 SPDSYN
-
 Leishmania donovani
4.1.1.50 AdoMetDC
-
 Leishmania donovani
4.1.1.50 S-Adenosylmethionine decarboxylase
-
 Leishmania donovani