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Literature summary extracted from

  • North, R.A.; Seizova, S.; Stampfli, A.; Kessans, S.A.; Suzuki, H.; Griffin, M.D.; Kvansakul, M.; Dobson, R.C.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine kinase from methicillin-resistant Staphylococcus aureus (2014), Acta Crystallogr. Sect. F, 70, 643-649.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.60 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.60 sitting drop vapor diffusion method, using 0.2 M ammonium chloride, 20% (w/v) polyethylene glycol 6000, 0.1 M HEPES pH 7.0 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.60 35000
-
 x * 35000, SDS-PAGE Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.60 ATP + N-acyl-D-mannosamine Staphylococcus aureus
-
 ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acyl-D-mannosamine Staphylococcus aureus USA300
-
 ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.60 Staphylococcus aureus
-
-
-
2.7.1.60 Staphylococcus aureus USA300
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.60 Q Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.60 ATP + N-acyl-D-mannosamine
-
 Staphylococcus aureus ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acyl-D-mannosamine
-
 Staphylococcus aureus USA300 ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.60 ? x * 35000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.60 nanK
-
 Staphylococcus aureus