Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sprenger, J.; Svensson, B.; Halander, J.; Carey, J.; Persson, L.; Al-Karadaghi, S.
    Three-dimensional structures of Plasmodium falciparum spermidine synthase with bound inhibitors suggest new strategies for drug design (2015), Acta Crystallogr. Sect. D, 71, 484-493.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expressed in Escherichia coli BL21 (lambdaDE3) Rosetta Oxford cells Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.16 in complex with S-methyl-5'-thioadenosine and putrescine or inhibitors, hanging drop vapor diffusion method, using 0.1 M MES buffer pH 5.6, 0.1 M ammonium sulfate, 27% (w/v) PEG 3350 Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 4-methylaniline
-
 Plasmodium falciparum
2.5.1.16 benzimidazol-(2-yl)pentan-1-amine
-
 Plasmodium falciparum
2.5.1.16 S-adenosyl-1,8-diamino-3-thio-octane
-
 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine Plasmodium falciparum
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Plasmodium falciparum Q8II73
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 Ni-affinity column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine
-
 Plasmodium falciparum S-methyl-5'-thioadenosine + spermidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 SPDS
-
 Plasmodium falciparum