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Literature summary extracted from

  • Qin, Z.; Xiao, Y.; Yang, X.; Mesters, J.R.; Yang, S.; Jiang, Z.
    A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain glycoside hydrolase family 3 beta-N-acetylglucosaminidase (2015), Sci. Rep., 5, 18292.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.3 expressed in Escherichia coli BL21-Gold (DE3) cells Rhizomucor miehei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.3 sitting drop vapor diffusion method, using 1.3-1.6 M (NH4)2SO4, 0.1 M Bis-Tris pH (5.5–6.5) at 20°C Rhizomucor miehei

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.3 H842A inactive Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.3 230000
-
 gel filtration Rhizomucor miehei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.3 acetyl-CoA + D-glucosamine Rhizomucor miehei
-
 CoA + N-acetyl-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.3 Rhizomucor miehei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.3 HisTrap column chromatography and Superdex 200 gel filtration Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.3 acetyl-CoA + chitobiose 77.5% activity compared to D-glucosamine Rhizomucor miehei CoA + N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
2.3.1.3 acetyl-CoA + chitopentaose 4.7% activity compared to D-glucosamine Rhizomucor miehei ?
-
 ?
2.3.1.3 acetyl-CoA + chitotetraose 9.3% activity compared to D-glucosamine Rhizomucor miehei ?
-
 ?
2.3.1.3 acetyl-CoA + chitotriose 12% activity compared to D-glucosamine Rhizomucor miehei ?
-
 ?
2.3.1.3 acetyl-CoA + D-glucosamine
-
 Rhizomucor miehei CoA + N-acetyl-D-glucosamine
-
 ?
2.3.1.3 acetyl-CoA + D-glucosamine 100% activity Rhizomucor miehei CoA + N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.3 dimer
-
 Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
2.3.1.3 GCN5-related glucosamine N-acetyltransferase
-
 Rhizomucor miehei
2.3.1.3 NAG
-
 Rhizomucor miehei