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Literature summary extracted from

  • Pade, N.; Linka, N.; Ruth, W.; Weber, A.P.; Hagemann, M.
    Floridoside and isofloridoside are synthesized by trehalose 6-phosphate synthase-like enzymes in the red alga Galdieria sulphuraria (2015), New Phytol., 205, 1227-1238.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.96 gene Gasu_26940, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree Galdieria sulphuraria
2.4.1.137 gene Gasu_10960, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree. Complementation of a salt-sensitive, glucosylglycerol-defective mutant of Synechocystis sp. PCC 6803 with Gasu_10960 Galdieria sulphuraria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.96 UDP-alpha-D-galactose + sn-glycerol 3-phosphate Galdieria sulphuraria
-
 UDP + 1-O-alpha-D-galactosyl-sn-glycerol 3-phosphate
-
 ?
2.4.1.96 UDP-alpha-D-galactose + sn-glycerol 3-phosphate Galdieria sulphuraria 074G
-
 UDP + 1-O-alpha-D-galactosyl-sn-glycerol 3-phosphate
-
 ?
2.4.1.137 UDP-alpha-D-galactose + sn-glycerol 3-phosphate Galdieria sulphuraria
-
 UDP + 2-(alpha-D-galactosyl)-sn-glycerol 3-phosphate
-
 ?
2.4.1.137 UDP-alpha-D-galactose + sn-glycerol 3-phosphate Galdieria sulphuraria 074G
-
 UDP + 2-(alpha-D-galactosyl)-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.96 Galdieria sulphuraria M2Y1U7 cf. EC 2.4.1.15; gene Gasu_26940
-
2.4.1.96 Galdieria sulphuraria 074G M2Y1U7 cf. EC 2.4.1.15; gene Gasu_26940
-
2.4.1.137 Galdieria sulphuraria
-
 gene Gasu_10960
-
2.4.1.137 Galdieria sulphuraria 074G
-
 gene Gasu_10960
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.96 additional information cells are grown in basal medium containing 25mM glucose as sole carbon source Galdieria sulphuraria
-
2.4.1.137 additional information cells are grown in basal medium containing 25mM glucose as sole carbon source Galdieria sulphuraria
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.96 additional information metabolite analysis by mass spectrometry Galdieria sulphuraria ?
-
 ?
2.4.1.96 additional information metabolite analysis by mass spectrometry Galdieria sulphuraria 074G ?
-
 ?
2.4.1.96 UDP-alpha-D-galactose + sn-glycerol 3-phosphate
-
 Galdieria sulphuraria UDP + 1-O-alpha-D-galactosyl-sn-glycerol 3-phosphate
-
 ?
2.4.1.96 UDP-alpha-D-galactose + sn-glycerol 3-phosphate
-
 Galdieria sulphuraria 074G UDP + 1-O-alpha-D-galactosyl-sn-glycerol 3-phosphate
-
 ?
2.4.1.137 additional information metabolite analysis by mass spectrometry Galdieria sulphuraria ?
-
 ?
2.4.1.137 additional information metabolite analysis by mass spectrometry Galdieria sulphuraria 074G ?
-
 ?
2.4.1.137 UDP-alpha-D-galactose + sn-glycerol 3-phosphate
-
 Galdieria sulphuraria UDP + 2-(alpha-D-galactosyl)-sn-glycerol 3-phosphate
-
 ?
2.4.1.137 UDP-alpha-D-galactose + sn-glycerol 3-phosphate
-
 Galdieria sulphuraria 074G UDP + 2-(alpha-D-galactosyl)-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.96 isofloridoside phosphate synthase/phosphatase fusion protein
-
 Galdieria sulphuraria
2.4.1.137 floridoside phosphate synthase/phosphatase fusion protein
-
 Galdieria sulphuraria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.96 30
-
 assay at Galdieria sulphuraria
2.4.1.137 30
-
 assay at Galdieria sulphuraria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.96 8
-
 assay at Galdieria sulphuraria
2.4.1.137 8
-
 assay at Galdieria sulphuraria

General Information

EC Number General Information Comment Organism
2.4.1.96 physiological function key enzyme isofloridoside phosphate synthase catalyzes the formation of isofloridoside phosphate from glycerol-3-phosphate and UDP-galactose. The intermediate is dephosphorylated by the isofloridoside phosphate phosphatase reaction to yield isofloridoside Galdieria sulphuraria
2.4.1.137 physiological function key enzyme floridoside phosphate synthase catalyzes the formation of floridoside phosphate from glycerol-3-phosphate and UDP-galactose. The intermediate is dephosphorylated by the floridoside phosphate phosphatase reaction to yield floridoside Galdieria sulphuraria