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Literature summary extracted from
- Molecular mechanisms of biocatalytic desulfurization of fossil fuels (1996), Nat. Biotechnol., 14, 1705-1709.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | additional information | no inhibition by EDTA | Rhodococcus erythropolis | |
| 1.14.14.22 | additional information | no inhibition by EDTA | Rhodococcus erythropolis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.21 | soluble | - |
Rhodococcus erythropolis | - |
- |
| 1.14.14.22 | soluble | - |
Rhodococcus erythropolis | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | additional information | the enzyme does not contain Fe2+ or Zn2+, no stimulation by Fe3+, Fe2+, or Cu2+, enzyme DszC does not require a metal cofactor | Rhodococcus erythropolis | |
| 1.14.14.22 | additional information | the enzyme does not contain Fe2+ or Zn2+, no stimulation by Fe3+, Fe2+, or Cu2+, enzyme DszA does not require a metal cofactor | Rhodococcus erythropolis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 44977 | - |
4 * 44977, sequence calculation | Rhodococcus erythropolis |
| 1.14.14.21 | 180000 | - |
gel filtration | Rhodococcus erythropolis |
| 1.14.14.22 | 49579 | - |
2 * 49579, sequence calculation | Rhodococcus erythropolis |
| 1.14.14.22 | 100000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | overall reaction | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |  |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | overall reaction | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + FMNH2 + O2 | Rhodococcus erythropolis | - |
dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + FMNH2 + O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene-5-oxide + FMNH2 + O2 | Rhodococcus erythropolis | - |
dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene-5-oxide + FMNH2 + O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | Rhodococcus erythropolis | A0SWL2 | gene dszC | - |
| 1.14.14.21 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | A0SWL2 | gene dszC | - |
| 1.14.14.22 | Rhodococcus erythropolis | - |
gene dszA | - |
| 1.14.14.22 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
gene dszA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | native enzyme from strain IGTS8 by dye ligand affinity chromatography, dialysis, and hydrophobic interaction chromatography, followed by gel filtration | Rhodococcus erythropolis |
| 1.14.14.22 | native enzyme from strain IGTS8 by anion exchange chromatography and gel filtration | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | overall reaction | Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | overall reaction | Rhodococcus erythropolis IGTS8 / ATCC 53968 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + FMNH2 + O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + FMNH2 + O2 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene-5-oxide + FMNH2 + O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene-5-oxide + FMNH2 + O2 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | homotetramer | 4 * 44977, sequence calculation | Rhodococcus erythropolis |
| 1.14.14.22 | homodimer | 2 * 49579, sequence calculation | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | DBT-MO | - |
Rhodococcus erythropolis |
| 1.14.14.21 | DBT-monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.21 | dszC | - |
Rhodococcus erythropolis |
| 1.14.14.22 | DBTO2-MO | - |
Rhodococcus erythropolis |
| 1.14.14.22 | DBTO2-monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.22 | dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 30 | - |
assay at | Rhodococcus erythropolis |
| 1.14.14.22 | 30 | - |
assay at | Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 7.5 | - |
assay at | Rhodococcus erythropolis |
| 1.14.14.22 | 7.5 | - |
assay at | Rhodococcus erythropolis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | FMNH2 | - |
Rhodococcus erythropolis | |
| 1.14.14.21 | NADH | the enzyme does not directly react with NADH, but uses the activity of the FMN:NADH oxidoreductase, DszD | Rhodococcus erythropolis | |
| 1.14.14.22 | FMNH2 | - |
Rhodococcus erythropolis | |
| 1.14.14.22 | NADH | the enzyme does not directly react with NADH, but uses the activity of the FMN:NADH oxidoreductase, DszD | Rhodococcus erythropolis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | metabolism | strain IGTS8 has the ability to convert dibenzothiophene to 2-hydroxybiphenyl with the release of inorganic sulfur. The conversion of dibenzothiophene to 2-hydroxybiphenyl is catalyzed by a multienzyme pathway consisting of two monooxygenases and a desulfinase. The final reaction catalyzed by the desulfinase DszB appears to be the rate limiting step in the pathway | Rhodococcus erythropolis |
| 1.14.14.22 | metabolism | strain IGTS8 has the ability to convert dibenzothiophene to 2-hydroxybiphenyl with the release of inorganic sulfur. The conversion of dibenzothiophene to 2-hydroxybiphenyl is catalyzed by a multienzyme pathway consisting of two monooxygenases and a desulfinase. The final reaction catalyzed by the desulfinase DszB appears to be the rate limiting step in the pathway | Rhodococcus erythropolis |
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