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Literature summary extracted from

  • Qiu, J.; Ma, Y.; Owusu, L.; Jiang, T.; Xin, Y.
    Functional analysis of serine acetyltransferase from Mycobacterium smegmatis (2014), J. Basic Microbiol., 54, 670-677.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 gene cysE or MSMEG_5947, DNA and amino acid sequence determination and analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 additional information generation of a MSMEG_5947 knockout mutant strain through homologous recombination, the mutant strain grows slowlier than the wild-type strain, and the lack of the CysE protein causes drastic morphological changes, overview Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 26200
-
 x * 26200, recombinant His-tagged enzyme, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Mycolicibacterium smegmatis
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Mycolicibacterium smegmatis ATCC 700084
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Mycolicibacterium smegmatis A0R4T4 gene cysE or MSMEG_5947
-
2.3.1.30 Mycolicibacterium smegmatis ATCC 700084 A0R4T4 gene cysE or MSMEG_5947
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Mycolicibacterium smegmatis CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Mycolicibacterium smegmatis ATCC 700084 CoA + O-acetyl-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.30 ? x * 26200, recombinant His-tagged enzyme, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 CysE
-
 Mycolicibacterium smegmatis
2.3.1.30 serine acetyltransferase
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
2.3.1.30 malfunction the MSMEG_5947 knockout mutant strain grows slower than the wild-type strain, and the lack of the CysE protein causes drastic morphological changes. Deletion of the serine acetyltransferase retards the growth of the organism, but serine acetyltransferase expression is not essential for the survival of the bacterium Mycolicibacterium smegmatis