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Literature summary extracted from

  • Ma, K.; Loessner, H.; Heider, J.; Johnson, M.K.; Adams, M.W.
    Effects of elemental sulfur on the metabolism of the deep-sea hyperthermophilic archaeon Thermococcus strain ES-1: characterization of a sulfur-regulated, non-heme iron alcohol dehydrogenase (1995), J. Bacteriol., 177, 4748-4756.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.014
-
 NADPH pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 0.05
-
 2-phenylacetaldehyde pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 0.25
-
 acetaldehyde pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 6.3
-
 NADP+ pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 8
-
 ethanol pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 15
-
 2-phenylethanol pH 8.0, 80°C Thermococcus paralvinellae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.2 cytoplasm
-
 Thermococcus paralvinellae 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.2 Iron enzyme contains 1 g-atom of Fe per subunit, present as a mixture of ferrous and ferric forms Thermococcus paralvinellae
1.1.1.2 additional information except for iron, no other metals are detected Thermococcus paralvinellae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 20000
-
 gel filtration Thermococcus paralvinellae
1.1.1.2 46000
-
 4 * 46000, SDS-PAGE Thermococcus paralvinellae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Thermococcus paralvinellae
-
-
-
1.1.1.2 Thermococcus paralvinellae ES-1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1-butanol + NADP+ 130% of the activity with ethanol Thermococcus paralvinellae butanal + NADPH + H+
-
 r
1.1.1.2 1-butanol + NADP+ 130% of the activity with ethanol Thermococcus paralvinellae ES-1 butanal + NADPH + H+
-
 r
1.1.1.2 1-heptanol + NADP+ 114% of the activity with ethanol Thermococcus paralvinellae heptanal + NADPH + H+
-
 r
1.1.1.2 1-hexanol + NADP+ 165% of the activity with ethanol Thermococcus paralvinellae hexanal + NADPH + H+
-
 r
1.1.1.2 1-pentanol + NADP+ 135% of the activity with ethanol Thermococcus paralvinellae pentanal + NADPH + H+
-
 r
1.1.1.2 1-propanol + NADP+ 102% of the activity with ethanol Thermococcus paralvinellae propanal + NADPH + H+
-
 r
1.1.1.2 2-phenylacetaldehyde + NADPH + H+
-
 Thermococcus paralvinellae 2-phenylethanol + NADP+
-
 r
1.1.1.2 2-phenylethanol + NADP+ 70% of the activity with ethanol Thermococcus paralvinellae 2-phenylacetaldehyde + NADPH + H+
-
 r
1.1.1.2 acetaldehyde + NADPH + H+
-
 Thermococcus paralvinellae ethanol + NADP+
-
 r
1.1.1.2 acetaldehyde + NADPH + H+
-
 Thermococcus paralvinellae ES-1 ethanol + NADP+
-
 r
1.1.1.2 ethanol + NADP+
-
 Thermococcus paralvinellae acetaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+
-
 Thermococcus paralvinellae ES-1 acetaldehyde + NADPH + H+
-
 r
1.1.1.2 isobutanol + NADP+ 93% of the activity with ethanol Thermococcus paralvinellae isobutanal + NADPH + H+
-
 r
1.1.1.2 isobutanol + NADP+ 93% of the activity with ethanol Thermococcus paralvinellae ES-1 isobutanal + NADPH + H+
-
 r
1.1.1.2 additional information enzyme utilizes NADP(H) as a cofactor and preferentially catalyzes aldehyde reduction Thermococcus paralvinellae ?
-
 ?
1.1.1.2 additional information enzyme utilizes NADP(H) as a cofactor and preferentially catalyzes aldehyde reduction Thermococcus paralvinellae ES-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 homotetramer 4 * 46000, SDS-PAGE Thermococcus paralvinellae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 ADH
-
 Thermococcus paralvinellae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 85
-
 half-life 85 h Thermococcus paralvinellae
1.1.1.2 95
-
 half-life 4 h Thermococcus paralvinellae
1.1.1.2 105
-
 melting temperature Thermococcus paralvinellae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADH about 0.1% of the activity with NADPH Thermococcus paralvinellae
1.1.1.2 NADPH
-
 Thermococcus paralvinellae

General Information

EC Number General Information Comment Organism
1.1.1.2 physiological function the specific activities of ADH, formate benzyl viologen oxidoreductase, and hydrogenase dramatically increase in cells grown under sulfur limitation. This is accompanied by increased amounts of H2 and alcohol (ethanol and butanol). Under S0 limitation, ADH may reduce to alcohols the aldehydes that are generated by fermentation, thereby serving to dispose of excess reductant Thermococcus paralvinellae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.2 2.1
-
 2-phenylethanol pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 6
-
 ethanol pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 76.4
-
 acetaldehyde pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 300
-
 2-phenylacetaldehyde pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 345
-
 NADPH pH 8.0, 80°C Thermococcus paralvinellae
1.1.1.2 2990
-
 NADP+ pH 8.0, 80°C Thermococcus paralvinellae