Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Engel, J.; Schmalhorst, P.S.; Krueger, A.T.; Mueller, C.T.; Buettner, F.F.; Routier, F.H.
    Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis (2015), Glycobiology, 25, 1423-1430.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.198 gene gtA, functional recombinant expression of FLAG-tagged GntA enzyme in Saccharomyces cerevisiae Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.198 Golgi apparatus
-
 Aspergillus fumigatus 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.198 40000
-
 x * 40000, recombinant FLAG-tagged GntA, SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.198 Aspergillus fumigatus
-
 gene gntA
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.198 glycoprotein two putative N-glycosylation sites at Asn66 and Asn243. The two predicted N-glycosylation sites are located on each side of the catalytically relevant DXD motif Aspergillus fumigatus

Subunits

EC Number Subunits Comment Organism
2.4.1.198 ? x * 40000, recombinant FLAG-tagged GntA, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.198 GntA
-
 Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.4.1.198 malfunction targeted deletion of the gene encoding GntA in A. fumigatus results in complete absence of zwitterionic glycoinositolphosphoceramide, a phenotype that can be reverted by episomal expression of GntA in the mutant Aspergillus fumigatus
2.4.1.198 physiological function the enzyme is involved in the initiation of zwitterionic glycoinositolphosphoceramide biosynthesis. Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids that, in fungi, commonly contain an alpha-mannose residue linked at position 2 of the inositol. Several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue at this position. In the human pathogen Aspergillus fumigatus, the alpha-N-acetylglucosaminyl-1,2-inositolphosphoceramide core is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant glycoinositolphosphoceramide synthesized by this fungus. GntA uses UDP-N-acetylglucosamine as donor substrate to generate a glycolipid product resistant to saponification and to digestion by phosphatidylinositol-phospholipase C as expected for GlcNAcalpha1,2IPC Aspergillus fumigatus