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Literature summary extracted from

  • Wu, S.; Liu, Y.; Yan, Q.; Jiang, Z.
    Gene cloning, functional expression and characterisation of a novel glycogen branching enzyme from Rhizomucor miehei and its application in wheat breadmaking (2014), Food Chem., 159, 85-94.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.18 food industry addition of RmGBE to wheat bread results in a 26% increase in specific volume and a 38% decrease in crumb firmness in comparison with the control. Besides, the retrogradation of bread is significantly retarded along with the enzyme reaction. These properties make RmGBE highly useful in the food and starch industries Rhizomucor miehei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 gene RmGBE, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 78200
-
 1 * 81030, sequence calculaiton, 1 * 78200, recombinant enzyme, SDS-PAGE Rhizomucor miehei
2.4.1.18 79600
-
 gel filtration, recombinant enzyme Rhizomucor miehei
2.4.1.18 81030
-
 1 * 81030, sequence calculaiton, 1 * 78200, recombinant enzyme, SDS-PAGE Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Rhizomucor miehei A0A059Q746 gene RmGBE
-
2.4.1.18 Rhizomucor miehei CAU432 A0A059Q746 gene RmGBE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Rhizomucor miehei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.18 10.7
-
 purified recombinant enzyme, pH 7.5, 25°C, substrate amylose Rhizomucor miehei

Subunits

EC Number Subunits Comment Organism
2.4.1.18 monomer 1 * 81030, sequence calculaiton, 1 * 78200, recombinant enzyme, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 GBE
-
 Rhizomucor miehei
2.4.1.18 glycogen branching enzyme
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 25
-
-
 Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.18 10 30 the enzyme displays excellent cold adaptation over a low temperature range of 10-30°C, retaining over 85% of its relative activity Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 10 40 purified recombinant enzyme, the enzyme retains about 80% of its activity at 40°C for 30 min, the enzyme displays excellent cold adaptation over a low temperature range of 10-30°C, retaining over 85% of its relative activity within this range Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 7.5
-
-
 Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.18 5.5 9.5 purified recombinant His-tagged enzyme, fairly stable over the broad pH range, retaining more than 80% of its activity Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.18 Rhizomucor miehei sequence calculation
-
 6.17