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Literature summary extracted from

  • Pohl, M.; Siegert, P.; Mesch, K.; Bruhn, H.; Groetzinger, J.
    Active site mutants of pyruvate decarboxylase from Zymomonas mobilis: A site-directed mutagenesis study of L112, I472, I476, E473, and N482 (1998), Eur. J. Biochem., 257, 538-546.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.1 E473D inactive Zymomonas mobilis
4.1.1.1 E473N inactive Zymomonas mobilis
4.1.1.1 I472A mutation influences the decarboxylation and carboligation reactions. The enlarged substrate-binding site allows the decarboxylation of longer aliphatic 2-keto acids (C4-C6) as well as aromatic 2-keto acids besides pyruvate, yielding hydroxypropiophenone, benzoin and phenylacetylcarbinol. Mutation impairs enantioselectivity Zymomonas mobilis
4.1.1.1 I476A mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
4.1.1.1 I476E mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
4.1.1.1 I476L mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
4.1.1.1 I476V mutation influences the decarboxylation and carboligation reactions and impairs enantioselectivity Zymomonas mobilis
4.1.1.1 N482D mutation has a significant influence on the carboligation reaction, the binding of the cofactors and the thermostability are not affected Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.1 1.1
-
 pyruvate wild-type, pH 6.5, 30°C Zymomonas mobilis
4.1.1.1 1.1
-
 pyruvate mutant L112A, pH 6.5, 30°C Zymomonas mobilis
4.1.1.1 3.4
-
 pyruvate mutant N482D, pH 6.5, 30°C Zymomonas mobilis
4.1.1.1 4.7
-
 pyruvate mutant I476L, pH 6.5, 30°C Zymomonas mobilis
4.1.1.1 6.8
-
 pyruvate mutant I476A, pH 6.5, 30°C Zymomonas mobilis
4.1.1.1 8.9
-
 pyruvate mutant I476V, pH 6.5, 30°C Zymomonas mobilis
4.1.1.1 9.1
-
 pyruvate mutant I472A, pH 6.5, 30°C Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.1 Zymomonas mobilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.1 pyruvate
-
 Zymomonas mobilis ethanal + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde wild-type 100% conversion, 98% enantiomeric excess, mutant I472A, 60% conversion, 70% enantiomeric excess, mutant I476E, 6% conversion, 60% enantiomeric excess of S-enantiomer Zymomonas mobilis (R)-phenylacetylcarbinol + CO2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.1 50
-
 mutant I476E, melting temperature Zymomonas mobilis
4.1.1.1 52
-
 mutant I476A, melting temperature Zymomonas mobilis
4.1.1.1 52
-
 mutant N482D, melting temperature Zymomonas mobilis
4.1.1.1 53
-
 mutant L112A, melting temperature Zymomonas mobilis
4.1.1.1 58
-
 mutant I476V, melting temperature Zymomonas mobilis
4.1.1.1 60
-
 mutant I476L, melting temperature Zymomonas mobilis
4.1.1.1 64
-
 wild-type, melting temperature Zymomonas mobilis