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Literature summary extracted from

  • Mordkovich, N.; Safonova, T.; Manuvera, V.; Veiko, V.; Polyakov, K.; Alekseev, K.; Mikhailov, S.; Popov, V.
    Physicochemical characterization of uridine phosphorylase from Shewanella oneidensis MR-1 (2013), Dokl. Biochem. Biophys., 451, 187-189.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 in complex with uridine, hanging drop vapor diffusion method, using Shewanella oneidensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 0.065
-
 5-methyluridine in 50 mM potassium phosphate buffer, pH 7.5, 25°C Shewanella oneidensis
2.4.2.3 0.082
-
 uridine in 50 mM potassium phosphate buffer, pH 7.5, 25°C Shewanella oneidensis
2.4.2.3 0.121
-
 2'-deoxyuridine in 50 mM potassium phosphate buffer, pH 7.5, 25°C Shewanella oneidensis
2.4.2.3 0.215
-
 thymidine in 50 mM potassium phosphate buffer, pH 7.5, 25°C Shewanella oneidensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 27000
-
 6 * 27000, SDS-PAGE Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Shewanella oneidensis
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate Shewanella oneidensis MR-1 / ATCC 700550
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Shewanella oneidensis C7EWM3
-
-
2.4.2.3 Shewanella oneidensis MR-1 / ATCC 700550 C7EWM3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 2'-deoxyuridine + phosphate
-
 Shewanella oneidensis uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.3 2'-deoxyuridine + phosphate
-
 Shewanella oneidensis MR-1 / ATCC 700550 uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.3 5-methyluridine + phosphate
-
 Shewanella oneidensis 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 5-methyluridine + phosphate
-
 Shewanella oneidensis MR-1 / ATCC 700550 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate
-
 Shewanella oneidensis thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate
-
 Shewanella oneidensis MR-1 / ATCC 700550 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Shewanella oneidensis uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Shewanella oneidensis MR-1 / ATCC 700550 uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.3 homohexamer 6 * 27000, SDS-PAGE Shewanella oneidensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.3 55
-
-
 Shewanella oneidensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 7.4
-
-
 Shewanella oneidensis