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Literature summary extracted from

  • Kobayashi, K.; Hattori, T.; Honda, Y.; Kirimura, K.
    Gene identification and functional analysis of methylcitrate synthase in citric acid-producing Aspergillus niger WU-2223L (2013), Biosci. Biotechnol. Biochem., 77, 1492-1498.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.5 expressed in Escherichia coli BL21(DE3) cells Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.5 mitochondrion
-
 Aspergillus niger 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.5 51300
-
 x * 51300, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Aspergillus niger
-
 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Aspergillus niger WU-2223L
-
 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Aspergillus niger
-
-
-
2.3.3.5 Aspergillus niger WU-2223L
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.5 HisTrap column chromatography Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 additional information the enzyme also exhibits citrate synthase activity Aspergillus niger ?
-
 ?
2.3.3.5 additional information the enzyme also exhibits citrate synthase activity Aspergillus niger WU-2223L ?
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate
-
 Aspergillus niger (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate
-
 Aspergillus niger WU-2223L (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.5 ? x * 51300, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 MCS
-
 Aspergillus niger
2.3.3.5 Methylcitrate synthase
-
 Aspergillus niger

General Information

EC Number General Information Comment Organism
2.3.3.5 metabolism the enzyme is essential in propionic acid metabolism Aspergillus niger