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Literature summary extracted from
- Cloning of a gene encoding flavin reductase coupling with dibenzothiophene monooxygenase through coexpression screening using indigo production as selective indication (2004), Biochem. Biophys. Res. Commun., 313, 570-575.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | gene bdsC, DNA and amino acid sequence determination and analysis, recombinant functional expression in Escherichia coli strain JM109, coexpression with a flavin reductase Frb, encoded by gene frb, UniProt ID Q75V96. Coexpression of frb with the DBT-desulfurization genes (bdsABC) from Bacillus subtilis strain WU-S2B is critical for high DBT-desulfurizing ability over a wide temperature range of 20-55°C, coexpression screening using indigo production as selective indication | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | Bacillus subtilis | Q8GRC5 | gene bdsC | - |
| 1.14.14.21 | Bacillus subtilis WU-S2B | Q8GRC5 | gene bdsC | - |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | DBT monooxygenase | - |
Bacillus subtilis |
| 1.14.14.21 | dibenzothiophene monooxygenase | - |
Bacillus subtilis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | physiological function | flavin reductase or flavin-dependent monooxygenase efficiently couples with the other component in two-component monooxygenases. Coexpression of frb with the DBT-desulfurization genes (bdsABC) from Bacillus subtilis strain WU-S2B is critical for high DBT-desulfurizing ability over a wide temperature range of 20-55°C | Bacillus subtilis |
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