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Literature summary extracted from

  • Murphy, J.R.; Donini, S.; Kappock, T.J.
    An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase (2015), Acta Crystallogr. Sect. F, 71, 1292-1299.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 expressed in Escherichia coli C41(DE3) cells Thermoplasma acidophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.16 hanging drop vapor diffusion method, using 0.1 M CHES pH 9.5, 12% (w/v) PEG 4000, 1 mM oxaloacetate Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 H222Q the mutant shows less than 0.05% of wild type activity Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 ACMX competitive inhibitor versus acetyl-CoA Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.003
-
 acetyl-CoA pH and temperature not specified in the publication Thermoplasma acidophilum
2.3.3.16 0.0052
-
 oxaloacetate pH and temperature not specified in the publication Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate Thermoplasma acidophilum
-
 citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 Ni2+-loaded iminodiacetic acid Sepharose column chromatography and Sephadex 200 gel filtration Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Thermoplasma acidophilum citrate + CoA
-
 ?