EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.47 | gene bioF, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.47 | purified recombinant enzyme, hanging-drop vapour diffusion, mixing of 0.002 ml of 5 mg/ml protein in 20 mM Tris-HCl pH 7.9, 100 mM NaCl, 2 mM DTT, with 0.002 ml of reservoir solution containing 150 mM DL-malic acid pH 7.4, 100 mM NaCl, 25% PEG 3350, equilibrqtion against 0.2 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution | Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 42000 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.47 | pimeloyl-[acyl-carrier protein] + L-alanine | Mycolicibacterium smegmatis | - |
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? | |
2.3.1.47 | pimeloyl-[acyl-carrier protein] + L-alanine | Mycolicibacterium smegmatis ATCC 700084 | - |
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.47 | Mycolicibacterium smegmatis | A0QX65 | gene bioF | - |
2.3.1.47 | Mycolicibacterium smegmatis ATCC 700084 | A0QX65 | gene bioF | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.47 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography, desalting gel filtration, and anion exchange chromatography followed by gel filtration, to over 95% purity | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.47 | pimeloyl-[acyl-carrier protein] + L-alanine | - |
Mycolicibacterium smegmatis | 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? | |
2.3.1.47 | pimeloyl-[acyl-carrier protein] + L-alanine | - |
Mycolicibacterium smegmatis ATCC 700084 | 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.47 | ? | x * 42000, recombinant His-tagged enzyme, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.47 | 7-keto-8-aminopelargonic acid synthase | - |
Mycolicibacterium smegmatis |
2.3.1.47 | BioF | - |
Mycolicibacterium smegmatis |
2.3.1.47 | KAPA synthase | - |
Mycolicibacterium smegmatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.47 | pyridoxal 5'-phosphate | dependent on | Mycolicibacterium smegmatis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.47 | metabolism | the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid | Mycolicibacterium smegmatis |