Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vail, A.; Wang, P.; Uefuji, H.; Samac, D.; Vance, C.; Wackett, L.; Sadowsky, M.
    Biodegradation of atrazine by three transgenic grasses and alfalfa expressing a modified bacterial atrazine chlorohydrolase gene (2015), Transgenic Res., 24, 475-488.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.8.1.8 environmental protection bioremidiation, use of enhanced expression of a modified bacterial atrazine chlorohydrolase, p-AtzA, in transgenic grasses, tall fescue or Festuca arundinacea, ryegrass or Lolium perenne, and switchgrass or Panicum virgatum, and the legume alfalfa, Medicago sativa, for the biodegradation of atrazine Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.8 gene atzA, transgenic expression in grasses, tall fescue, perennial ryegrass, and switchgrass, and the legume alfalfa or Medicago sativa, enhanced expression of p-AtzA is obtained by using combinations of the badnavirus promoter, the maize alcohol dehydrogenase first intron, and the maize ubiquitin promoter. For Medicago sativa, the first intron of the 5'-untranslated region tobacco alcohol dehydrogenase gene and the cassava vein mosaic virus promoter is used. Resistance of plants to atrazine in agar-based and hydroponic growth assays is correlated with in vivo levels of gene expression and atrazine degradation, trnasfection by Biolistic or by Agrobacterium tumefaciens methods, phenotypes, overview Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.8 atrazine + H2O Pseudomonas sp.
-
 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.8 Pseudomonas sp. P72156 gene atzA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.8 atrazine + H2O
-
 Pseudomonas sp. 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.8 AtzA
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
3.8.1.8 metabolism the enzyme is critical in the atrazine degradation pathway Pseudomonas sp.
3.8.1.8 physiological function Pseudomonas sp. strain ADP completely biodegrades atrazine to carbon dioxide and ammonia through the consecutive action of six catabolic enzymes, encoded by atzABCDEF, located on a selftransmissible plasmid, pADP-1. The first reaction is initiated by the enzyme AtzA, resulting in the dechlorination of atrazine to yield hydroxyatrazine, which is non-herbicidal and non-phytotoxic Pseudomonas sp.