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Literature summary extracted from

  • Lin, J.M.; Stark, B.C.; Webster, D.A.
    Effects of Vitreoscilla hemoglobin on the 2,4-dinitrotoluene (2,4-DNT) dioxygenase activity of Burkholderia and on 2,4-DNT degradation in two-phase bioreactors (2003), J. Ind. Microbiol. Biotechnol., 30, 362-368.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.12.24 Vitreoscilla hemoglobin 74% increase of activity at 0.04 mM Burkholderia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.24 0.04
-
 2,4-dinitrotoluene at pH 7.2 and 37°C Burkholderia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.12.24 cytosol
-
 Burkholderia sp. 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.24 171000
-
 calculated from amino acid sequence Burkholderia sp.
1.14.12.24 187000
-
 gel filtration Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2 Burkholderia sp.
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2 Burkholderia sp. DNT
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.24 Burkholderia sp.
-
-
-
1.14.12.24 Burkholderia sp. DNT
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.24 DEAE cellulose column chromatography and Sephadex G150 gel filtration Burkholderia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.24 19
-
 crude enzyme, at pH 7.2 and 37°C Burkholderia sp.
1.14.12.24 385
-
 after 20.3fold purification, at pH 7.2 and 37°C Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.24 2,4-DNT dioxygenase
-
 Burkholderia sp.