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Literature summary extracted from

  • Idkowiak-Baldys, J.; Takemoto, J.Y.; Grilley, M.M.
    Structure-function studies of yeast C-4 sphingolipid long chain base hydroxylase (2003), Biochim. Biophys. Acta, 1618, 17-24.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.5 D271T 98% of wild-type activity Saccharomyces cerevisiae
1.14.18.5 F174L complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H176A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H180A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H190A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H193A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H194A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H249A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H270A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H273A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 H274A complete loss of activity Saccharomyces cerevisiae
1.14.18.5 L196W complete loss of activity Saccharomyces cerevisiae
1.14.18.5 additional information the replacement of any one of conserved His residues of three histidine-rich motifs with an alanine eliminates hydroxylase activity in vivo and in vitro. Residues Phe 174, Asn 182, Ser 191, Leu 196, Pro 199, Asn 266, Tyr 269, Asp 271 and Gln 275 appear to be additionally important elements of the active site but their conversion into corresponding yeast DELTA7-sterol-C5(6)-desaturase Erg3p residues does not lead to a gain in desaturase activity Saccharomyces cerevisiae
1.14.18.5 N182P complete loss of activity Saccharomyces cerevisiae
1.14.18.5 N266G complete loss of activity Saccharomyces cerevisiae
1.14.18.5 P199C complete loss of activity Saccharomyces cerevisiae
1.14.18.5 Q275L complete loss of activity Saccharomyces cerevisiae
1.14.18.5 S191K 99% of wild-type activity Saccharomyces cerevisiae
1.14.18.5 Y269C complete loss of activity Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.5 Saccharomyces cerevisiae P38992
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.5 dihydrosphingosine + NADPH + H+ + O2
-
 Saccharomyces cerevisiae phytosphingosine + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.5 sphingolipid C4-hydroxylase
-
 Saccharomyces cerevisiae
1.14.18.5 SUR2
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.14.18.5 physiological function C-4 hydroxylation of sphingolipids is important for the antifungal action of syringomycin E. Mutants with defects in C-4 hydroxylated sphingoid base biosynthesis are resistant to this antifungal agent Saccharomyces cerevisiae