EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.251 | molecular dynamics simulation and comparison of Salmonella typhimurium and Escherichia coli enzymes. In vitro lipase activity of the Salmonella enzyme is 15-20fold higher than for the Escherichia coli enzyme. Protein stability correlates inversely with activity: the Escherichia coli PagP equilibrium free energy is 2fold higher | Salmonella enterica subsp. enterica serovar Typhimurium |
2.3.1.251 | molecular dynamics simulation and comparison of Salmonella typhimurium and Escherichia coli enzymes. In vitro lipase activity of the Salmonella enzyme is 15-20fold higher than for the Escherichia coli enzyme. Protein stability correlates inversely with activity: the Escherichia coli PagP equilibrium free energy is 2fold higher | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.251 | K91S | mutation leads to 2fold reduction in the protein stability by about 3.45 kcal/mol | Salmonella enterica subsp. enterica serovar Typhimurium |
2.3.1.251 | L57Q | mutation leads to destabilizization by about 3.5 kcal/mol | Escherichia coli |
2.3.1.251 | Q57L | presence of Leu confers an added 2.2 kcal/mol stabilization | Salmonella enterica subsp. enterica serovar Typhimurium |
2.3.1.251 | S91K | mutation leads to stabilizization by about 3.0 kcal/mol | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.251 | Escherichia coli | P37001 | - |
- |
2.3.1.251 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |