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Literature summary extracted from

  • Wright, B.S.; Snow, J.W.; OBrien, T.C.; Lynch, D.V.
    Synthesis of 4-hydroxysphinganine and characterization of sphinganine hydroxylase activity in corn (2003), Arch. Biochem. Biophys., 415, 184-192.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.5 0.033
-
 NADPH pH 6.5, 30°C Zea mays
1.14.18.5 0.058
-
 NADH pH 6.5, 30°C Zea mays
1.14.18.5 0.06
-
 D-erythro-sphinganine pH 6.5, 30°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.5 endoplasmic reticulum associated with endoplasmic reticulum Zea mays 5783
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.5 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.5 shoot
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.5 D-erythro-eicosasphinganine + NADH + H+ + O2
-
 Zea mays 4-hydroxyeicosasphinganine + NAD+ + H2O
-
 ?
1.14.18.5 D-erythro-sphinganine + NADH + H+ + O2
-
 Zea mays 4-hydroxysphinganine + NAD+ + H2O free 4-hydroxysphinganine is produced in excised corn shoots by the direct hydroxylation of sphinganine and not from the breakdown of complex sphingolipids ?
1.14.18.5 D-erythro-sphinganine + NADPH + H+ + O2
-
 Zea mays 4-hydroxysphinganine + NADP+ + H2O
-
 ?
1.14.18.5 additional information DL-threo-sphinganine is not an effective substrate. Individual ceramide species having 2-, 6-,8-, or 24-carbon fatty acyl chains amide-linked to sphinganine are hydroxylated with similar efficiency as free sphinganine Zea mays ?
-
 ?
1.14.18.5 N-acetylsphinganine + NADPH + H+ + O2
-
 Zea mays N-acetyl-4-hydroxysphinganine + NADP+ + H2O
-
 ?
1.14.18.5 N-hexanoylsphinganine + NADPH + H+ + O2
-
 Zea mays N-hexanoyl-4-hydroxysphinganine + NADP+ + H2O
-
 ?
1.14.18.5 N-octanoylsphinganine + NADPH + H+ + O2
-
 Zea mays N-octanoyl-4-hydroxysphinganine + NADP+ + H2O
-
 ?

Expression

EC Number Organism Comment Expression
1.14.18.5 Zea mays sphinganine hydroxylase activity in microsomes isolated from corn shoots treated with fumonisin B1 increases more than 3fold compared to controls up