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Literature summary extracted from
- Mutation study of conserved amino acid residues of Spirulina DELTA6-acyl-lipid desaturase showing involvement of histidine 313 in the regioselectivity of the enzyme (2004), Appl. Microbiol. Biotechnol., 66, 74-84.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.46 | expressed in Escherichia coli DH5alpha cells | Arthrospira platensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.19.46 | D138N | inactive | Arthrospira platensis |
| 1.14.19.46 | E140Q | inactive | Arthrospira platensis |
| 1.14.19.46 | G136H | inactive | Arthrospira platensis |
| 1.14.19.46 | H124R | inactive | Arthrospira platensis |
| 1.14.19.46 | H128R | inactive | Arthrospira platensis |
| 1.14.19.46 | H129R | inactive | Arthrospira platensis |
| 1.14.19.46 | H305R | the mutant shows 17% of wild type activity | Arthrospira platensis |
| 1.14.19.46 | H306R | inactive | Arthrospira platensis |
| 1.14.19.46 | H313R | inactive | Arthrospira platensis |
| 1.14.19.46 | H315N | inactive | Arthrospira platensis |
| 1.14.19.46 | H89R | inactive | Arthrospira platensis |
| 1.14.19.46 | H93R | the mutant shows 11% of wild type activity | Arthrospira platensis |
| 1.14.19.46 | R123N | the mutant shows 91% of wild type activity | Arthrospira platensis |
| 1.14.19.46 | W294G | inactive | Arthrospira platensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.46 | 0.5 | - |
linoleic acid | wild type enzyme, at pH 7.5 and 25°C | Arthrospira platensis |  |
| 1.14.19.46 | 0.5 | - |
linoleic acid | mutant enzyme R123N, at pH 7.5 and 25°C | Arthrospira platensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.46 | 47000 | - |
x * 47000, SDS-PAGE | Arthrospira platensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.46 | linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arthrospira platensis | - |
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.46 | Arthrospira platensis | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.46 | linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arthrospira platensis | gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.19.46 | ? | x * 47000, SDS-PAGE | Arthrospira platensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.46 | DELTA6-acyl-lipid desaturase | - |
Arthrospira platensis |
| 1.14.19.46 | desD | - |
Arthrospira platensis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.46 | Ferredoxin | - |
Arthrospira platensis |
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Release 2023.1 (January 2023)
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