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Literature summary extracted from

  • Hsu, F.; Zhu, W.; Brennan, L.; Tao, L.; Luo, Z.Q.; Mao, Y.
    Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase (2012), Proc. Natl. Acad. Sci. USA, 109, 13567-13572.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.67 expressed in Escherichia coli Legionella pneumophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.67 crystal structures of SidF and its complex with bound short-chain (dibutanoyl) derivative of PI(3,4)P2 is shown. The structures show that the conserved CX5R catalytic motif is located in a large cationic groove Legionella pneumophila

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.67 C645S inactive mutant Legionella pneumophila
3.1.3.67 additional information deletion of the C-terminal portion of SidF including the two transmembrane motifs changes its localization to the cell periphery Legionella pneumophila
3.1.3.67 additional information deletion of the putative transmembrane domains does not affect its enzymatic activity Legionella pneumophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.67 additional information ectopically expressed GFP-SidF localizes to the ER membrane in mammalian cells. Deletion of the C-terminal portion of SidF including the two transmembrane motifs changes its localization to the cell periphery Legionella pneumophila
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.67 Legionella pneumophila Q5ZSD5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.67 phosphatidylinositol 3,4,5-trisphosphate + H2O
-
 Legionella pneumophila phosphatidylinositol 4,5-bisphosphate + phosphate
-
 ?
3.1.3.67 phosphatidylinositol 3,4-bisphosphate + H2O preferred substrate Legionella pneumophila phosphatidylinositol 4-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.67 SidF
-
 Legionella pneumophila

General Information

EC Number General Information Comment Organism
3.1.3.67 malfunction deletion of SidF results in the reduced recruitment of effector proteins that anchor on the Legionella-containing vacuole (LCV) via binding to PI(4)P Legionella pneumophila