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Literature summary extracted from

  • Fan, X.; Liu, X.; Huang, R.; Liu, Y.
    Identification and characterization of a novel thermostable pyrethroid-hydrolyzing enzyme isolated through metagenomic approach (2012), Microb. Cell Fact., 11, 33.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.88 expressed in Escherichia coli as a His-tagged fusion protein Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.88 0.0141
-
 4-nitrophenyl acetate pH 6.5, 55°C Escherichia coli
3.1.1.88 0.0316
-
 4-nitrophenyl butyrate pH 6.5, 55°C Escherichia coli
3.1.1.88 0.0332
-
 4-nitrophenyl caprylate pH 6.5, 55°C Escherichia coli
3.1.1.88 0.0576
-
 4-nitrophenyl caproate pH 6.5, 55°C Escherichia coli
3.1.1.88 0.0997
-
 4-nitrophenyl decanoate pH 6.5, 55°C Escherichia coli
3.1.1.88 0.13
-
 4-nitrophenyl laurate pH 6.5, 55°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.88 30800
-
 calculated from cDNA Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.88 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.88 using Ni-NTA chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.88 49.1
-
 substrate: 4-nitrophenyl laurate, pH 6.5, 55°C Escherichia coli
3.1.1.88 69.1
-
 substrate: 4-nitrophenyl decanoate, pH 6.5, 55°C Escherichia coli
3.1.1.88 88.9
-
 substrate: 4-nitrophenyl caproate, pH 6.5, 55°C Escherichia coli
3.1.1.88 149.7
-
 substrate: 4-nitrophenyl caprylate, pH 6.5, 55°C Escherichia coli
3.1.1.88 163.3
-
 substrate: 4-nitrophenyl butyrate, pH 6.5, 55°C Escherichia coli
3.1.1.88 772.9
-
 substrate: 4-nitrophenyl acetate, pH 6.5, 55°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.1.88 25°C,10 weeks, 32% loss of activity Escherichia coli
3.1.1.88 4°C, 10 weeks, 6% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.88 4-nitrophenyl acetate + H2O
-
 Escherichia coli 4-nitrophenol + acetate
-
 ?
3.1.1.88 4-nitrophenyl butyrate + H2O
-
 Escherichia coli 4-nitrophenol + butyrate
-
 ?
3.1.1.88 4-nitrophenyl caproate + H2O
-
 Escherichia coli 4-nitrophenol + caproate
-
 ?
3.1.1.88 4-nitrophenyl caprylate + H2O
-
 Escherichia coli 4-nitrophenol + caprylate
-
 ?
3.1.1.88 4-nitrophenyl decanoate + H2O
-
 Escherichia coli 4-nitrophenol + decanoate
-
 ?
3.1.1.88 4-nitrophenyl laurate + H2O
-
 Escherichia coli 4-nitrophenyl + laurate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.88 Sys410
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.88 55
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.88 50
-
 enzyme ist stable at temperatures below 50°C Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.88 49.1
-
 4-nitrophenyl laurate pH 6.5, 55°C Escherichia coli
3.1.1.88 69.1
-
 4-nitrophenyl decanoate pH 6.5, 55°C Escherichia coli
3.1.1.88 88.9
-
 4-nitrophenyl caproate pH 6.5, 55°C Escherichia coli
3.1.1.88 149.7
-
 4-nitrophenyl caprylate pH 6.5, 55°C Escherichia coli
3.1.1.88 163.3
-
 4-nitrophenyl butyrate pH 6.5, 55°C Escherichia coli
3.1.1.88 289.1
-
 4-nitrophenyl acetate pH 6.5, 55°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.88 6.5
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.88 4.5 8.5
-
 Escherichia coli