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Literature summary extracted from

  • Honda, H.; Nagaoka, S.; Kawai, Y.; Kemperman, R.; Kok, J.; Yamazaki, Y.; Tateno, Y.; Kitazawa, H.; Saito, T.
    Purification and characterization of two phospho-?-galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323 (2012), J. Gen. Appl. Microbiol., 58, 11-17.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.85 LacG1, phylogenetic analysis Lactobacillus gasseri
3.2.1.85 LacG2, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Lactococcus lactis strain MG1363 Lactobacillus gasseri
3.2.1.86 LacG1, phylogenetic analysis Lactobacillus gasseri
3.2.1.86 LacG2, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Lactococcus lactis strain MG1363 Lactobacillus gasseri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 3.8
-
 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate pH 6.0, 40°C Lactobacillus gasseri
3.2.1.85 5.5
-
 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate pH 5.5, 50°C Lactobacillus gasseri
3.2.1.86 0.66
-
 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri
3.2.1.86 0.97
-
 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 6.0, 50°C Lactobacillus gasseri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.85 55000
-
 x * 55000, recombinant enzyme, SDS-PAGE Lactobacillus gasseri
3.2.1.86 55000
-
 x * 55000, recombinant enzyme, SDS-PAGE Lactobacillus gasseri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Lactobacillus gasseri O06532
-
-
3.2.1.85 Lactobacillus gasseri ATCC 33323 O06532
-
-
3.2.1.86 Lactobacillus gasseri O06532
-
-
3.2.1.86 Lactobacillus gasseri ATCC 33323 O06532
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85 native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration Lactobacillus gasseri
3.2.1.85 recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration Lactobacillus gasseri
3.2.1.86 native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration Lactobacillus gasseri
3.2.1.86 recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration Lactobacillus gasseri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactobacillus gasseri 2-nitrophenol + beta-D-galactose 6-phosphate
-
 ?
3.2.1.85 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactobacillus gasseri ATCC 33323 2-nitrophenol + beta-D-galactose 6-phosphate
-
 ?
3.2.1.85 additional information the enzyme also shows 6-phospho-beta-glucosidase activity, EC 3.2.1.86 Lactobacillus gasseri ?
-
 ?
3.2.1.85 additional information the enzyme also shows 6-phospho-beta-glucosidase activity, EC 3.2.1.86 Lactobacillus gasseri ATCC 33323 ?
-
 ?
3.2.1.86 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Lactobacillus gasseri 2-nitrophenol + beta-D-glucose 6-phosphate
-
 ?
3.2.1.86 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Lactobacillus gasseri ATCC 33323 2-nitrophenol + beta-D-glucose 6-phosphate
-
 ?
3.2.1.86 additional information the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85 Lactobacillus gasseri ?
-
 ?
3.2.1.86 additional information the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85 Lactobacillus gasseri ATCC 33323 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.85 ? x * 55000, recombinant enzyme, SDS-PAGE Lactobacillus gasseri
3.2.1.86 ? x * 55000, recombinant enzyme, SDS-PAGE Lactobacillus gasseri

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 LacG1
-
 Lactobacillus gasseri
3.2.1.85 LacG2
-
 Lactobacillus gasseri
3.2.1.86 LacG1
-
 Lactobacillus gasseri
3.2.1.86 LacG2
-
 Lactobacillus gasseri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.85 40
-
-
 Lactobacillus gasseri
3.2.1.85 50
-
-
 Lactobacillus gasseri
3.2.1.86 40
-
-
 Lactobacillus gasseri
3.2.1.86 50
-
-
 Lactobacillus gasseri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.85 206
-
 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate pH 6.0, 40°C Lactobacillus gasseri
3.2.1.85 361
-
 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate pH 5.5, 50°C Lactobacillus gasseri
3.2.1.86 2 8 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri
3.2.1.86 41
-
 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 6.0, 50°C Lactobacillus gasseri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.85 5.5
-
-
 Lactobacillus gasseri
3.2.1.85 6
-
-
 Lactobacillus gasseri
3.2.1.86 6
-
-
 Lactobacillus gasseri
3.2.1.86 7
-
-
 Lactobacillus gasseri

General Information

EC Number General Information Comment Organism
3.2.1.85 metabolism the enzyms is involved in the metabolic pathway of lactose in Lactbacillus gasseri, overview Lactobacillus gasseri
3.2.1.86 metabolism the enzyme is involved in the metabolic pathway of lactose in Lactobacillus gasseri, overview Lactobacillus gasseri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.85 56
-
 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate pH 6.0, 40°C Lactobacillus gasseri
3.2.1.85 93
-
 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate pH 6.0, 40°C Lactobacillus gasseri
3.2.1.86 44
-
 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri
3.2.1.86 59
-
 2-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.0, 40°C Lactobacillus gasseri