Literature summary extracted from
Zhou, W.; Ding, W.L.; Zeng, X.L.; Dong, L.L.; Zhao, B.; Zhou, M.; Scheer, H.; Zhao, K.H.; Yang, X.
Structure and mechanism of the phycobiliprotein lyase CpcT (2014), J. Biol. Chem., 289, 26677-26689.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.4.1.30 |
overexpression in Escherichia coli BL21(DE3) |
Nostoc sp. |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.4.1.30 |
crystal structure of the apoenzyme and its complex with phycocyanobilin at 1.95 and 2.50 A resolution |
Nostoc sp. |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.4.1.30 |
D163A |
lyase activity is less than 5% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
D163V |
lyase activity is less than 5% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
D63N |
lyase activity is about 70% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
E111Q |
lyase activity is about 75% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
K87M |
lyase activity is about 70% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
M118A |
lyase activity is about 25% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
N83A |
lyase activity is about 35% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
Q55A |
lyase activity is about 30% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
R141I |
lyase activity is about 15% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
R68A |
lyase activity is about 10% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
S150A |
lyase activity is about 125% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
S161V |
lyase activity is about 105% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
Y59A |
lyase activity is about 35% compared to wild-type enzyme |
Nostoc sp. |
4.4.1.30 |
Y65F |
lyase activity is less than 5% compared to wild-type enzyme |
Nostoc sp. |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.4.1.30 |
Nostoc sp. |
Q8YLF9 |
- |
- |
4.4.1.30 |
Nostoc sp. PCC 7120 |
Q8YLF9 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.4.1.30 |
- |
Nostoc sp. |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.4.1.30 |
dimer |
at the concentration of 0.003 mM, the enzyme is mostly monomeric in the absence of phycocyanobilin, but it tends to dimerize in the presence of 0.006 mM phycocyanobilin |
Nostoc sp. |
4.4.1.30 |
monomer |
at the concentration of 0.003 mM, the enzyme is mostly monomeric in the absence of phycocyanobilin, but it tends to dimerize in the presence of 0.006 mM phycocyanobilin |
Nostoc sp. |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.4.1.30 |
phycobiliprotein lyase CpcT |
- |
Nostoc sp. |