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Literature summary extracted from

  • Biswas, A.; Boutaghou, M.; Alvey, R.; Kronfel, C.; Cole, R.; Bryant, D.; Schluchter, W.
    Characterization of the activities of the CpeY, CpeZ, and CpeS bilin lyases in phycoerythrin biosynthesis in Fremyella diplosiphon strain UTEX 481 (2011), J. Biol. Chem., 286, 35509-35521.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.29 expression in Escherichia coli Microchaete diplosiphon
4.4.1.30 expression in Escherichia coli Microchaete diplosiphon

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.29 Microchaete diplosiphon
-
-
-
4.4.1.29 Microchaete diplosiphon Q9ALZ8
-
-
4.4.1.30 Microchaete diplosiphon Q9ALZ8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.29 apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
-
 Microchaete diplosiphon [phycoerythrin alpha-subunit]-Cys82-phycoerythrobilin CpeS bilin lyase ligates phycoerythrobilin at both Cys82 and Cys139 of phycoerythrin alpha-subunit, but very inefficiently ?
4.4.1.29 apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
-
 Microchaete diplosiphon [phycoerythrin alpha-subunit]-Cys82-phycoerythrobilin ligase subunit CpeY alone, but not subunit CpeZ, can ligate phycoerythrobilin to phycoerythrin alpha-subunit, but the yield of phycoerythrin alpha-subunit-phycoerythrobilin is lower than achieved with subunits CpeY and CpeZ together ?
4.4.1.29 apo-[phycoerythrin beta-subunit] + phycoerythrobilin
-
 Microchaete diplosiphon [phycoerythrin beta-subunit]-Cys80-phycoerythrobilin CpeS bilin lyase efficiently ligates phycoerythrobilin to Cys80 of phycoerythrin beta-subunit ?
4.4.1.29 additional information neither subunit CpeY, subunit CpeZ, nor CpeY/CpeZ can ligate phycoerythrobilin to phycoerythrin beta-subunit Microchaete diplosiphon ?
-
 ?
4.4.1.30 apo-[phycoerythrin alpha-subunit] + phycoerythrobilin
-
 Microchaete diplosiphon [phycoerythrin beta-subunit]-Cys82-phycoerythrobilin CpeS bilin lyase ligates phycoerythrobilin at both Cys82 and Cys139 of phycoerythrin alpha-subunit, but very inefficiently ?