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Literature summary extracted from

  • Mootz, J.M.; Malone, C.L.; Shaw, L.N.; Horswill, A.R.
    Staphopains modulate Staphylococcus aureus biofilm integrity (2013), Infect. Immun., 81, 3227-3238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.48 expressed in Escherichia coli strain ER2566 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.48 C237A inactive ScpA mutant Staphylococcus aureus
3.4.22.48 C243A inactive SspB mutant Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.48 E-64
-
 Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.48 extracellular
-
 Staphylococcus aureus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.48 21000
-
 x * 21000, ScpA, SDS-PAGE Staphylococcus aureus
3.4.22.48 22000
-
 x * 22000, SspB, SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus aureus
-
-
-
3.4.22.48 Staphylococcus aureus AH1263
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.48 His-Select nickel affinity column chromatography Staphylococcus aureus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.48 using 50 mM sodium phosphate, 150 mM sodium chloride, 1 mM EDTA, 0.5 M arginine (pH 6.0), 30% (v/v) glycerol, 1 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.48 5-carboxyfluorescein-Lys-Lys-Ala-Ala-Glu-Ala-Ser-Lys-(QXL520)-OH + H2O substrate for ScpA Staphylococcus aureus ?
-
 ?
3.4.22.48 5-carboxyfluorescein-Lys-Lys-Ala-Ala-Glu-Ala-Ser-Lys-(QXL520)-OH + H2O substrate for ScpA Staphylococcus aureus AH1263 ?
-
 ?
3.4.22.48 Bz-Pro-Phe-Arg-4-nitroanilide + H2O substrate for SspB Staphylococcus aureus ?
-
 ?
3.4.22.48 Bz-Pro-Phe-Arg-4-nitroanilide + H2O substrate for SspB Staphylococcus aureus AH1263 ?
-
 ?
3.4.22.48 elastin agar + H2O
-
 Staphylococcus aureus ?
-
 ?
3.4.22.48 elastin agar + H2O
-
 Staphylococcus aureus AH1263 ?
-
 ?
3.4.22.48 milk agar + H2O
-
 Staphylococcus aureus ?
-
 ?
3.4.22.48 milk agar + H2O
-
 Staphylococcus aureus AH1263 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.48 ? x * 21000, ScpA, SDS-PAGE Staphylococcus aureus
3.4.22.48 ? x * 22000, SspB, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.48 SCPA
-
 Staphylococcus aureus
3.4.22.48 SspB
-
 Staphylococcus aureus

Expression

EC Number Organism Comment Expression
3.4.22.48 Staphylococcus aureus staphopain levels are decreased under biofilm-forming conditions up

General Information

EC Number General Information Comment Organism
3.4.22.48 physiological function staphopains SspB and ScpA modulate Staphylococcus aureus biofilm integrity. ScpA enzyme has a conserved ability to inhibit biofilm formation Staphylococcus aureus