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Literature summary extracted from

  • Hakki, Z.; Thompson, A.J.; Bellmaine, S.; Speciale, G.; Davies, G.J.; Williams, S.J.
    Structural and kinetic dissection of the endo-alpha-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp. (2015), Chemistry, 21, 1966-1977.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.130 wild type and mutant enzyme E333Q, in complex with 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside or alpha-mannopyranosyl-(1->3)-isofagomine, hanging drop vapor diffusion method, using 2.8 M sodium acetate pH 6.4 Bacteroides xylanisolvens
3.2.1.198 in complex with inhibitor alpha-mannopyranosyl-1,3-isofagomine. The inhibitor is coordinated by residues Y46, W48, H63, W126, E156, E333 and E336 Bacteroides xylanisolvens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.130 E333Q inactive Bacteroides xylanisolvens
3.2.1.198 E333Q inactive Bacteroides xylanisolvens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.130 alpha-mannopyranosyl-(1->3)-isofagomine
-
 Bacteroides xylanisolvens
3.2.1.130 alpha-mannopyranosyl-1->3-isofagomine
-
 Bacteroides thetaiotaomicron
3.2.1.198 alpha-mannopyranosyl-1,3-isofagomine Kd value 140 nm Bacteroides thetaiotaomicron
3.2.1.198 alpha-mannopyranosyl-1,3-isofagomine Kd value 140 nm Bacteroides xylanisolvens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.130 0.038
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
3.2.1.130 0.404
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
3.2.1.130 1.827
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron
3.2.1.198 0.038
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
3.2.1.198 0.404
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
3.2.1.198 1.83
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron
3.2.1.198 2.6
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.130 Bacteroides thetaiotaomicron
-
-
-
3.2.1.130 Bacteroides xylanisolvens
-
-
-
3.2.1.198 Bacteroides thetaiotaomicron Q8A109 GH99 family protein
-
3.2.1.198 Bacteroides thetaiotaomicron DSM 2079 Q8A109 GH99 family protein
-
3.2.1.198 Bacteroides xylanisolvens D6D1V7 GH99 family protein
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.130 (3R,4R,5R)-3-(alpha-D-mannopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)-piperidine + H2O
-
 Bacteroides thetaiotaomicron D-mannose + (3R,4R,5R)-3,4-dihydroxy-5-hydroxymethylpiperidine
-
 ?
3.2.1.130 (alpha-Man-(1->3)-isofagomine) + H2O
-
 Bacteroides xylanisolvens D-mannose + isofagomine
-
 ?
3.2.1.130 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.130 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
3.2.1.130 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.130 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
3.2.1.130 alpha-mannan + H2O
-
 Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.130 alpha-mannan + H2O
-
 Bacteroides xylanisolvens ?
-
 ?
3.2.1.130 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron alpha-(1->3)-D-mannobiose + methyl alpha-(1->2)-D-mannobioside + ?
-
 ?
3.2.1.130 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens alpha-(1->3)-D-mannobiose + methyl alpha-(1->2)-D-mannobioside + ?
-
 ?
3.2.1.198 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens 4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
3.2.1.198 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
3.2.1.198 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
3.2.1.198 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
3.2.1.198 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
3.2.1.198 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
 ?
3.2.1.198 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides xylanisolvens alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
-
 ?
3.2.1.198 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
-
 ?
3.2.1.198 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
-
 ?
3.2.1.198 additional information enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose Bacteroides xylanisolvens ?
-
 ?
3.2.1.198 additional information enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.198 additional information enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose Bacteroides thetaiotaomicron DSM 2079 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.130 endo-alpha-1,2-mannanase
-
 Bacteroides xylanisolvens
3.2.1.130 endo-alpha-1->2-mannanase
-
 Bacteroides thetaiotaomicron
3.2.1.130 GH99
-
 Bacteroides thetaiotaomicron
3.2.1.130 GH99
-
 Bacteroides xylanisolvens
3.2.1.198 BXY_34140
-
 Bacteroides xylanisolvens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.130 0.0000717
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
3.2.1.130 0.00008
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
3.2.1.130 0.00115
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron
3.2.1.198 0.0007
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
3.2.1.198 0.0008
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
3.2.1.198 0.0015
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron
3.2.1.198 3
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron

General Information

EC Number General Information Comment Organism
3.2.1.198 physiological function enzyme releases alpha-1,3-mannobiose by hydrolyzing alphaMan-1,3-aMan->1,2-alphaMan-1,2-alphaMan sequences within branches off the main alpha-1,6-mannan backbone of fungal cell walls Bacteroides xylanisolvens
3.2.1.198 physiological function enzyme releases alpha-1,3-mannobiose by hydrolyzing alphaMan-1,3-aMan->1,2-alphaMan-1,2-alphaMan sequences within branches off the main alpha-1,6-mannan backbone of fungal cell walls Bacteroides thetaiotaomicron

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.130 0.208
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
3.2.1.130 1.78
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides xylanisolvens
3.2.1.130 6.3
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron
3.2.1.130 102.7
-
 4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C Bacteroides thetaiotaomicron
3.2.1.198 0.00062
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron
3.2.1.198 0.0021
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
3.2.1.198 0.0085
-
 4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides xylanisolvens
3.2.1.198 1.15
-
 methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside pH 7, 37°C Bacteroides thetaiotaomicron