Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ishino, S.; Makita, N.; Shiraishi, M.; Yamagami, T.; Ishino, Y.
    EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furiosus (2015), Biochimie, 118, 264-269.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.B1 overexpression in Escherichia coli Pyrococcus furiosus
3.1.21.7 overexpression in Escherichia coli Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.B1 47653
-
 x * 47653, calculated from sequence Pyrococcus furiosus
3.1.21.7 23580
-
 x * 23580, calculated from sequence, His-tagged enzyme Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.B1 deoxyinosine-containing deoxyoligonucleotide + H2O Pyrococcus furiosus the enzyme is involved in damaged DNA repair ?
-
 ?
3.1.21.7 deoxyinosine-containing deoxyoligonucleotide + H2O Pyrococcus furiosus the enzyme is involved in damaged DNA repair ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.B1 Pyrococcus furiosus Q8U0N5
-
-
3.1.21.7 Pyrococcus furiosus Q8U263
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.B1
-
 Pyrococcus furiosus
3.1.21.7
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.B1 deoxyinosine-containing deoxyoligonucleotide + H2O the enzyme is involved in damaged DNA repair Pyrococcus furiosus ?
-
 ?
3.1.21.B1 deoxyinosine-containing deoxyoligonucleotide + H2O endonucleolytic cleavage of the phosphodiester bond 5' from deoxyinosine Pyrococcus furiosus ?
-
 ?
3.1.21.7 deoxyinosine-containing deoxyoligonucleotide + H2O the enzyme is involved in damaged DNA repair Pyrococcus furiosus ?
-
 ?
3.1.21.7 deoxyinosine-containing deoxyoligonucleotide + H2O the enzyme hydrolyzes the second phosphodiester bond 3' from deoxyinosine Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.B1 ? x * 47653, calculated from sequence Pyrococcus furiosus
3.1.21.7 ? x * 23580, calculated from sequence, His-tagged enzyme Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.1.21.B1 EndoQ
-
 Pyrococcus furiosus
3.1.21.B1 PF1551 locus name Pyrococcus furiosus
3.1.21.B1 PfuEndoQ
-
 Pyrococcus furiosus
3.1.21.7 EndoV
-
 Pyrococcus furiosus
3.1.21.7 PF0987 locus name Pyrococcus furiosus
3.1.21.7 PfuEndoV
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.B1 75
-
-
 Pyrococcus furiosus
3.1.21.7 75
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.21.B1 70 80 70°C: about 50% of maximal activity, 80°C: about 35% of maximal activity Pyrococcus furiosus
3.1.21.7 70 80 70°C: about 50% of maximal activity, 80°C: about 35% of maximal activity Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.B1 9.5
-
-
 Pyrococcus furiosus
3.1.21.7 8.5
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.B1 8.5 9.5 pH 8.5: about 60% of maximal activity, pH 9.5: optimum, pH 10.0: less than 19% of maximal activity Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.21.B1 Pyrococcus furiosus calculated from sequence
-
 8.1
3.1.21.7 Pyrococcus furiosus calculated from sequence
-
 9.8

General Information

EC Number General Information Comment Organism
3.1.21.B1 physiological function the enzyme is involved in damaged DNA repair. EndoQ predominately functions over EndoV (EC 3.1.21.7) to start the process of damaged base repair in Pyrococcus furiosus cells Pyrococcus furiosus
3.1.21.7 physiological function the enzyme is involved in damaged DNA repair Pyrococcus furiosus