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Literature summary extracted from

  • Zhou, P.; Chen, Z.; Yan, Q.; Yang, S.; Hilgenfeld, R.; Jiang, Z.
    The structure of a glycoside hydrolase family 81 endo-?-1,3-glucanase. (2013), Acta Crystallogr. Sect. D, 69, 2027-2038.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged wild-type and selenomethionine-labeled enzyme in Escherichia coli Rhizomucor miehei
3.2.1.39 expression in Escherichia coli Rhizomucor miehei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.39 purified recombinant detagged wild-type and selenomethionine-labeled enzymes, sitting drop vapour diffusion method, mixing of 0.001 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, with 0.001 ml of precipitant solution containing 200 mM Li2SO4, 30% w/v PEG 4000, 100 mM Tris-HCl, pH 8.5, or 160 mM Li2SO4, 24% w/v PEG 4000, 80 mM Tris-HCl, pH 8.5, 6% v/v 2-methyl-2,4-pentanediol, 2-4 weeks, X-ray diffraction structure determination and analysis at 2.0-2.3 A resolution Rhizomucor miehei
3.2.1.39 the overall structure of the Lam81A monomer consists of an N-terminal beta-sandwich domain, a C-terminal (alpha/alpha)6 domain and an additional domain between them. Residues Glu553 and Glu557 are proposed to serve as the proton donor and basic catalyst, respectively, in a single-displacement mechanism. In addition, Tyr386, Tyr482 and Ser554 possibly contribute to both the position or the ionization state of the basic catalyst Glu557 Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Rhizomucor miehei A0A023I7E1 gene RmLam81A
-
3.2.1.39 Rhizomucor miehei U5HK45
-
-
3.2.1.39 Rhizomucor miehei CAU432 A0A023I7E1 gene RmLam81A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli by nickel affinity chromatography, ultrafiltration, tag cleavage by Bacillus subtilis proteases, and gel filtration Rhizomucor miehei

Subunits

EC Number Subunits Comment Organism
3.2.1.39 More the enzyme monomer consists of an N-terminal beta-sandwich domain, a C-terminal (alpha/alpha)6 domain and an additional domain between them, three-dimensional structure of enzyme RmLam81A domain A, domain B and domain C, overview Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
 Rhizomucor miehei
3.2.1.39 GH family 81 beta-1,3-glucanase
-
 Rhizomucor miehei
3.2.1.39 Lam81A
-
 Rhizomucor miehei
3.2.1.39 laminarinase
-
 Rhizomucor miehei

General Information

EC Number General Information Comment Organism
3.2.1.39 evolution the enzyme belongs to the glycosyl hydrolase family 81, GH81, but has a unique structure Rhizomucor miehei
3.2.1.39 additional information the enzyme monomer consists of an N-terminal beta-sandwich domain, a C-terminal (alpha/alpha)6 domain and an additional domain between them. Glu553 and Glu557 are proposed to serve as the proton donor and basic catalyst, respectively, in a single-displacement mechanism. In addition, Tyr386, Tyr482 and Ser554 possibly contribute to both the position or the ionization state of the basic catalyst Glu557, catalytic cleft and the active site structure, overview Rhizomucor miehei