Literature summary extracted from

Yang, Y.; Ma, J.; Yang, Y.; Zhang, X.; Wang, Y.; Yang, L.; Sun, M.
Yeast 3,5-bisphosphate nucleotidase: an affinity tag for protein purification (2014), Protein Expr. Purif., 97, 81-87.

Application

EC Number	Application	Comment	Organism
3.1.3.7	synthesis	construction of a protein expression vector using 3',5'-bisphosphate nucleotidase HAL2 in the presence of Ca2+ as an affinity tag. The tag can be used for procaryotic protein expression and purification by maintaining Ca2+ for efficient affinity binding and chelating Ca2+ for elution	Saccharomyces cerevisiae

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.3.7	expression in Escherichia coli	Saccharomyces cerevisiae

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.3.7	Ca2+	competitive to Mg2+. Inhibition by Ca2+can be prevented by addition of Mg2+	Saccharomyces cerevisiae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.3.7	0.00025	-	3'-phosphoadenosine 5'-phosphate	pH 8.0, temperature not specified in the publication	Saccharomyces cerevisiae

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.7	Saccharomyces cerevisiae	P32179	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.3.7	recombinant protein	Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.7	3'-phosphoadenosine 5'-phosphate + H2	-	Saccharomyces cerevisiae	AMP + phosphate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.3.7	Hal2	-	Saccharomyces cerevisiae
3.1.3.7	met22	-	Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1.3.7	11	-	3'-phosphoadenosine 5'-phosphate	pH 8.0, temperature not specified in the publication	Saccharomyces cerevisiae

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
3.1.3.7	0.035	-	pH 8.0, temperature not specified in the publication	Saccharomyces cerevisiae	Ca2+

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)