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Literature summary extracted from

  • Sousa, S.A.; Feliciano, J.R.; Pinheiro, P.F.; Leitao, J.H.
    Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-D-mannose 4,6-dehydratase (2013), PLoS ONE, 8, e56902.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.47 expressed in Escherichia coli BL21(DE3) cells Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.47 GDP-alpha-D-mannose substrate inhibition Burkholderia cepacia
4.2.1.47 Mg2+ high concentrations of MgCl2 (25 mM) have an inhibitory effect on enzyme activity Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.47 1.024
-
 GDP-alpha-D-mannose at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.47 Mg2+ required for activity, the enzyme inactive in its absence Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.47 162000
-
 gel filtration Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.47 GDP-alpha-D-mannose Burkholderia cepacia
-
 GDP-4-dehydro-alpha-D-rhamnose + H2O
-
 ?
4.2.1.47 GDP-alpha-D-mannose Burkholderia cepacia J2315
-
 GDP-4-dehydro-alpha-D-rhamnose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.47 Burkholderia cepacia B4EFL2
-
-
4.2.1.47 Burkholderia cepacia J2315 B4EFL2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.47 Hi-Trap chelating column chromatography Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.47 GDP-alpha-D-mannose
-
 Burkholderia cepacia GDP-4-dehydro-alpha-D-rhamnose + H2O
-
 ?
4.2.1.47 GDP-alpha-D-mannose
-
 Burkholderia cepacia J2315 GDP-4-dehydro-alpha-D-rhamnose + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.47 tetramer
-
 Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
4.2.1.47 bceN
-
 Burkholderia cepacia
4.2.1.47 GDP-D-mannose-4,6-dehydratase
-
 Burkholderia cepacia
4.2.1.47 GMD
-
 Burkholderia cepacia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.47 1
-
 GDP-alpha-D-mannose at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.47 additional information unaffected by NAD+ or NADP+ Burkholderia cepacia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.47 2.913
-
 GDP-alpha-D-mannose at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein Burkholderia cepacia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.47 0.977
-
 GDP-alpha-D-mannose at pH 7.4 and 25°C, in the presence of 1 mol MgCl2 per mol of protein Burkholderia cepacia