EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.5 | gene GLX-I or LOC_Os08g09250, functional complementation of Saccaromyces cerevisiae GLY I mutant DELTAGLO I, heterologous expression in Escherichia coli and Nicotiana tabacum resulting in improved adaptation to various abiotic stresses caused by increased scavenging of methylglyoxal, lower Na+/K+ ratio and maintenance of reduced glutathione levels in the hosts | Oryza sativa Japonica Group |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.4.1.5 | E145Q | the mutant shows altered thermodynamic parameters for Ni2+ ion binding compared to the wild-type enzyme | Oryza sativa Japonica Group |
4.4.1.5 | E209D | inactive mutant | Oryza sativa Japonica Group |
4.4.1.5 | E78Q | the mutant shows altered thermodynamic parameters for Ni2+ ion binding compared to the wild-type enzyme | Oryza sativa Japonica Group |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | EDTA | strong inhibition | Oryza sativa Japonica Group |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 0.0998 | - |
(R)-S-lactoylglutathione | recombinant enzyme, pH 7.2, temperature not specified in the publication | Oryza sativa Japonica Group |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | Co2+ | results in about 25% of the activity with Ni2+ | Oryza sativa Japonica Group | |
4.4.1.5 | Mg2+ | only slight activation | Oryza sativa Japonica Group | |
4.4.1.5 | additional information | no activating effect by Zn2+, Ca2+, Fe2+, and Mn2+ | Oryza sativa Japonica Group | |
4.4.1.5 | Ni2+ | dependent on, required for activity, Glu145 and Glu209 are involved in Ni2+ binding, Glu78 is not involved, the monomeric enzyme possesses a single Ni2+ coordination site despite containing two GLY I domains, 0.052 mol/mol enzyme for the recombinant enzyme expressed in Escherichia coli, and 1.258 mol/mol enzyme after exogenous addition of Ni2+ | Oryza sativa Japonica Group |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 34900 | - |
recombinant enzyme, gel filtration | Oryza sativa Japonica Group |
4.4.1.5 | 36000 | - |
1 * 36000, recombinant enzyme, SDS-PAGE | Oryza sativa Japonica Group |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | glutathione + methylglyoxal | Oryza sativa Japonica Group | - |
(R)-S-lactoylglutathione | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.5 | Oryza sativa Japonica Group | Q948T6 | subsp. japonica, gene GLX-I or LOC_Os08g09250 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.4.1.5 | leaf | - |
Oryza sativa Japonica Group | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 8.3 | - |
purified recombinant enzyme without metal ion, pH 7.2, temperature not specified in the publication | Oryza sativa Japonica Group |
4.4.1.5 | 120 | - |
purified recombinant enzyme with Ni2+ addition, pH 7.2, temperature not specified in the publication | Oryza sativa Japonica Group |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | (R)-S-lactoylglutathione | - |
Oryza sativa Japonica Group | glutathione + methylglyoxal | - |
r | |
4.4.1.5 | glutathione + methylglyoxal | - |
Oryza sativa Japonica Group | (R)-S-lactoylglutathione | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.5 | monomer | 1 * 36000, recombinant enzyme, SDS-PAGE | Oryza sativa Japonica Group |
4.4.1.5 | More | homology-based structural modeling, overview | Oryza sativa Japonica Group |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.5 | Gly I | - |
Oryza sativa Japonica Group |
4.4.1.5 | glyoxalase I | - |
Oryza sativa Japonica Group |
4.4.1.5 | OsGLYI-11.2 | - |
Oryza sativa Japonica Group |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 70.96 | - |
(R)-S-lactoylglutathione | recombinant enzyme, pH 7.2, temperature not specified in the publication | Oryza sativa Japonica Group |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 7.2 | 7.3 | - |
Oryza sativa Japonica Group |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 5.5 | 8 | high activity at, recombinant enzyme | Oryza sativa Japonica Group |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.4.1.5 | Oryza sativa Japonica Group | the enzyme is highly substrate-inducible | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.4.1.5 | metabolism | the glyoxalase system constitutes the major pathway for the detoxification of metabolically produced cytotoxin methylglyoxal into a non-toxic metabolite D-lactate | Oryza sativa Japonica Group |
4.4.1.5 | additional information | homology-based structural modeling, overview | Oryza sativa Japonica Group |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 7100 | - |
(R)-S-lactoylglutathione | recombinant enzyme, pH 7.2, temperature not specified in the publication | Oryza sativa Japonica Group |