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Literature summary extracted from

  • Filimonova, M.; Gubskaya, V.; Nuretdinov, I.
    Some features of hydrolysis of the hybrid B-Z-form dna by Serratia marcescens nuclease (2014), OnLine J. Biol. Sci., 14, 179-185.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 Mg2+ binding of Co(NH3)6 3+ to highly polymerized DNA caused correspondingly about 7fold decrease in Km and more than 600fold decrease in Kcat compared with that of Mg-DNA complex of B-form; binding of Mg2+ to highly polymerized DNA causes correspondingly about 80fold decrease in Km and more than 1600fold decrease in Kcat compared with that of Mg-DNA complex of B-form Serratia marcescens
3.1.30.2 additional information DNA transition from the right handed B-form to the hybrid B-Z-form causes a decrease in Vmax, Km and Kcat of DNA cleavage with the nuclease Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.30.2 additional information
-
 additional information kinetics of the enzyme with differently structured DNA substrate and different metal ions, overview Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.2 additional information Serratia marcescens the enzyme potently degrades both DNA and RNA and is a nuclease with broad specificity, but it shows some sensitivity to the secondary structure of the substrate ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Serratia marcescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.2 B-form DNA + H2O B form of herring testis DNA Serratia marcescens ?
-
 ?
3.1.30.2 B-Z-hybrid form DNA + H2O hybrid B-Z form of herring testis DNA Serratia marcescens ?
-
 ?
3.1.30.2 additional information the enzyme potently degrades both DNA and RNA and is a nuclease with broad specificity, but it shows some sensitivity to the secondary structure of the substrate Serratia marcescens ?
-
 ?
3.1.30.2 additional information substrate is highly polymerized herring testis DNA of the random nucleotide sequence. Hydrolysis of the B-form and hybrid B-Z form DNA by the enzyme. The hybrid B-Z form is formed upon addition of MgSO4 and Co(NH3)6Cl3 Serratia marcescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 Serratia marcescens nuclease
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 Serratia marcescens
3.1.30.2 Sma nuc
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 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.2 30
-
 assay at Serratia marcescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.30.2 0.44
-
 B-Z-hybrid form DNA pH 8.0, 30°C, in presence of Mg2+ Serratia marcescens
3.1.30.2 1.2
-
 B-Z-hybrid form DNA pH 8.0, 30°C, in presence of Co(NH3)6 3+ Serratia marcescens
3.1.30.2 737.6
-
 B-form DNA pH 8.0, 30°C, in presence of Mg2+ Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.2 8
-
 assay at Serratia marcescens