Literature summary extracted from
Jiang, X.; Chen, P.; Yin, M.; Yang, Q.
Constitutive expression, purification and characterisation of pectin methylesterase from Aspergillus niger in Pichia pastoris for potential application in the fruit juice industry (2013), J. Sci. Food Agric., 93, 375-381.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.11 |
expressed in Pichia pastoris strain GS115 |
Aspergillus niger |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.1.11 |
food industry |
fruit juice industry |
Aspergillus niger |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.1.11 |
Co2+ |
52% residual activity at 100 mM |
Aspergillus niger |
|
3.1.1.11 |
K+ |
73% residual activity at 100 mM |
Aspergillus niger |
|
3.1.1.11 |
Mg2+ |
86% residual activity at 100 mM |
Aspergillus niger |
|
3.1.1.11 |
Mn2+ |
61% residual activity at 100 mM |
Aspergillus niger |
|
3.1.1.11 |
Ni2+ |
47% residual activity at 100 mM |
Aspergillus niger |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.1.11 |
additional information |
not affected by Na+ |
Aspergillus niger |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.1.11 |
45000 |
- |
x * 45000, SDS-PAGE |
Aspergillus niger |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.1.11 |
pectin + H2O |
Aspergillus niger |
- |
methanol + pectate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.11 |
Aspergillus niger |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.1.1.11 |
glycoprotein |
- |
Aspergillus niger |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.1.11 |
ammonium sulfate precipitation, HiTrap Q column chromatography, and Resource S column chromatography |
Aspergillus niger |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.1.11 |
9.67 |
- |
supernatant, at pH 4.7 and 50°C |
Aspergillus niger |
3.1.1.11 |
342 |
- |
after 35.4fold purification, at pH 4.7 and 50°C |
Aspergillus niger |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.1.11 |
pectin + H2O |
- |
Aspergillus niger |
methanol + pectate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.1.11 |
? |
x * 45000, SDS-PAGE |
Aspergillus niger |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.11 |
pectin methylesterase |
- |
Aspergillus niger |
3.1.1.11 |
PME |
- |
Aspergillus niger |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.1.11 |
50 |
- |
- |
Aspergillus niger |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.1.1.11 |
35 |
60 |
90-100% activity from 35-50°C, about 30% activity at 55°C, no activity at 60°C |
Aspergillus niger |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.1.11 |
4.7 |
- |
- |
Aspergillus niger |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.1.1.11 |
4.5 |
5 |
about 90% activity at pH 4.5,4.6, and 4.8, 100% activity at pH 4.7, about 60% activity at pH 4.9, about 50% activity at pH 5.0 |
Aspergillus niger |